A novel, selective radioligand, [125I]-[Lys5, Tyr(I2)7, Meleu9, Nle10]-NKA(4-10), for the tachykinin NK-2 receptor

Tim Badgery-Parker, Xiang-Ping Zeng, Solange Lavielle, Elizabeth Burcher*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A new radioligand, [125I]-[Lys5Tyr(I2)7,MeLeu9,Nle10]-NKA(4-10), based on the selective agonist [Lys5,MeLeu9,Nle10]-NKA(4-10) has been developed. Binding in rat fundus membranes was displaced by NPγ>NKA≥[Lys5,MeLeu9,Nle10]-NKA(4-10)>neuropeptide K> Lys5,Tyr I2MeLeu9,Nle10]-NKA(4-10)>SP> [Sar9,Met(O211]-SP≫ senktide, indicating binding to NK-2 receptors. Preliminary studies demostrated high specific binding in membranes from rat urinary bladder, duodenum and colon. Specific binding in rat brain and lung was negligible, and binding in a range of guinea-pig tissues was no more than 35% specific. These data may indicate species differences in NK-2 receptors.

Original languageEnglish
Pages (from-to)455-457
Number of pages3
JournalRegulatory Peptides
Volume46
Issue number1-2
DOIs
Publication statusPublished - 2 Jul 1993
Externally publishedYes

Keywords

  • Neuropeptide γ
  • NK-2 receptor
  • Rat gastric fundus
  • Selective radioligand
  • Tachykinin

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