A portable nucleic acid detection system using natural convection combined with a smartphone

Vinoth Kumar Rajendran, Padmavathy Bakthavathsalam, Peter L. Bergquist, Anwar Sunna

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The development of portable nucleic acid diagnostic devices has the potential to expand the availability of molecular diagnostics into low-resource settings. One of the promising solutions for rapid and simple DNA amplification is the use of Rayleigh–Bernard natural convection which is caused by a buoyancy-driven thermal gradient of liquid when heated from below. This natural convection avoids the use of the complex and sophisticated hardware that is required for precise maintenance of temperature cycles in conventional PCR. We have developed a stand-alone convective PCR (cPCR) device linked to a smartphone for rapid detection of nucleic acids using natural convection heating. The device amplifies multiple DNA samples simultaneously using a custom-made heat block controlled by Bluetooth wireless communication. The entire device is highly portable, user-friendly, battery-operated and can provide target DNA amplification in less than 30 min. A detection limit of 2.8 × 103 copies of a segment of lambda DNA was obtained when the two different fluorescently-tagged amplicons were collected magnetically and detected using the smartphone fluorescence reader. Thus, the combination of cPCR and multiplex fluorescence-based detection on a smartphone provides new opportunities for the development of affordable and portable molecular diagnostic devices for point-of-care situations or remote clinical settings.
LanguageEnglish
Pages68-75
Number of pages8
JournalBiosensors and Bioelectronics
Volume134
DOIs
Publication statusPublished - 1 Jun 2019

Fingerprint

Convection
Smartphones
Nucleic acids
Natural convection
Nucleic Acids
DNA
Equipment and Supplies
Molecular Pathology
Amplification
Fluorescence
Hot Temperature
Point-of-Care Systems
Bluetooth
Buoyancy
Polymerase Chain Reaction
Thermal gradients
Multiplex Polymerase Chain Reaction
Heating
Availability
Limit of Detection

Keywords

  • Convective PCR
  • Multiplex detection
  • Smartphone
  • Magnetic separation
  • Point-of-care
  • Diagnostics

Cite this

Rajendran, Vinoth Kumar ; Bakthavathsalam, Padmavathy ; Bergquist, Peter L. ; Sunna, Anwar. / A portable nucleic acid detection system using natural convection combined with a smartphone. In: Biosensors and Bioelectronics. 2019 ; Vol. 134. pp. 68-75.
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A portable nucleic acid detection system using natural convection combined with a smartphone. / Rajendran, Vinoth Kumar; Bakthavathsalam, Padmavathy; Bergquist, Peter L.; Sunna, Anwar.

In: Biosensors and Bioelectronics, Vol. 134, 01.06.2019, p. 68-75.

Research output: Contribution to journalArticleResearchpeer-review

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