A processed multidomain Mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen, and swine respiratory cilia

Lisa M. Seymour, Ania T. Deutscher, Cheryl Jenkins, Tracey A. Kuit, Linda Falconer, F. Chris Minion, Ben Crossett, Matthew P. Padula, Nicholas E. Dixon, Steven P. Djordjevic, Mark J. Walker*

*Corresponding author for this work

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Porcine enzootic pneumonia is a chronic respiratory disease that affects swine. The etiological agent of the disease, Mycoplasma hyopneumoniae, is a bacterium that adheres to cilia of the swine respiratory tract, resulting in loss of cilia and epithelial cell damage. A M. hyopneumoniae protein P116, encoded by mhp108, was investigated as a potential adhesin. Examination of P116 expression using proteomic analyses observed P116 as a full-length protein and also as fragments, ranging from 17 to 70 kDa in size. A variety of pathogenic bacterial species have been shown to bind the extracellular matrix component fibronectin as an adherence mechanism. M. hyopneumoniae cells were found to bind fibronectin in a dose-dependent and saturable manner. Surface plasmon resonance was used to show that a recombinant C-terminal domain of P116 bound fibronectin at physiologically relevant concentrations (K(D) 24 +/- 6 nM). Plasmin(ogen)-binding proteins are also expressed by many bacterial pathogens, facilitating extracellular matrix degradation. M. hyopneumoniae cells were found to also bind plasminogen in a dose-dependent and saturable manner; the C-terminal domain of P116 binds to plasminogen (K(D) 44 +/- 5 nM). Plasminogen binding was abolished when the C-terminal lysine of P116 was deleted, implicating this residue as part of the plasminogen binding site. P116 fragments adhere to the PK15 porcine kidney epithelial-like cell line and swine respiratory cilia. Collectively these data suggest that P116 is an important adhesin and virulence factor of M. hyopneumoniae.

Original languageEnglish
Pages (from-to)33971-33978
Number of pages8
JournalJournal of Biological Chemistry
Volume285
Issue number44
DOIs
Publication statusPublished - 29 Oct 2010
Externally publishedYes

Keywords

  • STREPTOCOCCAL M-PROTEIN
  • ELONGATION-FACTOR TU
  • ALPHA-ENOLASE
  • SPF PIGLETS
  • ADHERENCE
  • CELLS
  • GENE
  • PIGS
  • IDENTIFICATION
  • PNEUMONIA

Cite this

Seymour, L. M., Deutscher, A. T., Jenkins, C., Kuit, T. A., Falconer, L., Minion, F. C., ... Walker, M. J. (2010). A processed multidomain Mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen, and swine respiratory cilia. Journal of Biological Chemistry, 285(44), 33971-33978. https://doi.org/10.1074/jbc.M110.104463