A proteomics view on the role of drought-induced senescence and oxidative stress defense in enhanced stem reserves remobilization in wheat

Mitra Mohammadi Bazargani, Elham Sarhadi, Ali-Akbar Shahnejat Bushehri, Andrea Matros, Hans Peter Mock, Mohammad Reza Naghavi, Vahid Hajihoseini, Mohsen Mardi, Mohammad-Reza Hajirezaei, Foad Moradi, Bahman Ehdaie, Ghasem Hosseini Salekdeh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

101 Citations (Scopus)

Abstract

Drought is one of the major factors limiting the yield of wheat (Triticum aestivum L.) particularly during grain filling. Under terminal drought condition, remobilization of pre-stored carbohydrates in wheat stem to grain has a major contribution in yield. To determine the molecular mechanism of stem reserve utilization under drought condition, we compared stem proteome patterns of two contrasting wheat landraces (N49 and N14) under a progressive post-anthesis drought stress, during which period N49 peduncle showed remarkably higher stem reserves remobilization efficiency compared to N14. Out of 830 protein spots reproducibly detected and analyzed on two-dimensional electrophoresis gels, 135 spots showed significant changes in at least one landrace. The highest number of differentially expressed proteins was observed in landrace N49 at 20. days after anthesis when active remobilization of dry matter was observed, suggesting a possible involvement of these proteins in effective stem reserve remobilization of N49. The identification of 82 of differentially expressed proteins using mass spectrometry revealed a coordinated expression of proteins involved in leaf senescence, oxidative stress defense, signal transduction, metabolisms and photosynthesis which might enable N49 to efficiently remobilized its stem reserves compared to N14. The up-regulation of several senescence-associated proteins and breakdown of photosynthetic proteins in N49 might reflect the fact that N49 increased carbon remobilization from the stem to the grains by enhancing senescence. Furthermore, the up-regulation of several oxidative stress defense proteins in N49 might suggest a more effective protection against oxidative stress during senescence in order to protect stem cells from premature cell death. Our results suggest that wheat plant might response to soil drying by efficiently remobilize assimilates from stem to grain through coordinated gene expression.

Original languageEnglish
Pages (from-to)1959-1973
Number of pages15
JournalJournal of Proteomics
Volume74
Issue number10
DOIs
Publication statusPublished - 6 Sep 2011
Externally publishedYes

Keywords

  • Drought
  • Grain filling
  • MALDI-TOF
  • MALDI-TOF/TOF
  • Oxidative stress
  • Q-TOF
  • Remobilization
  • Senescence
  • Stem
  • Stem reserves
  • Two-dimensional electrophoresis
  • Wheat

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