A pulse radiolysis investigation of the reactions of myeloperoxidase with superoxide and hydrogen peroxide

Anthony J. Kettle; David F. Sangster; Janusz M. Gebicki; Christine C. Winterbourn

doi:10.1016/0167-4838(88)90297-X

A pulse radiolysis investigation of the reactions of myeloperoxidase with superoxide and hydrogen peroxide

Anthony J. Kettle^*, David F. Sangster, Janusz M. Gebicki, Christine C. Winterbourn

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Using pulse radiolysis, the rate constant for the reaction of ferric myeloperoxidase with O₂^- to give compound III was measured at pH 7.8, and values of 2.1 · 10⁶ M^-1 · s^-1 for equine ferric myeloperoxidase and 1.1 · 10⁶ M^-1 · s^-1 for human ferric myeloperoxidase were obtained. Under the same conditions, the rate constant for the reaction of human ferric myeloperoxidase with H₂O₂ to give compound I was 3.1 · 10⁷ M^-1 · s^-1. Our results indicate that although the reaction of ferric myeloperoxidase with O₂^- is an order of magnitude slower than with H₂O₂, the former reaction is sufficiently rapid to influence myeloperoxidase-dependent production of hypochlorous acid by stimulated neutrophils.

Original language	English
Pages (from-to)	58-62
Number of pages	5
Journal	Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume	956
Issue number	1
DOIs	https://doi.org/10.1016/0167-4838(88)90297-X
Publication status	Published - 31 Aug 1988

Keywords

Hydrogen peroxide
Myeloperoxidase
Pulse radiolysis
Superoxide

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10.1016/0167-4838(88)90297-X

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title = "A pulse radiolysis investigation of the reactions of myeloperoxidase with superoxide and hydrogen peroxide",

abstract = "Using pulse radiolysis, the rate constant for the reaction of ferric myeloperoxidase with O2- to give compound III was measured at pH 7.8, and values of 2.1 · 106 M-1 · s-1 for equine ferric myeloperoxidase and 1.1 · 106 M-1 · s-1 for human ferric myeloperoxidase were obtained. Under the same conditions, the rate constant for the reaction of human ferric myeloperoxidase with H2O2 to give compound I was 3.1 · 107 M-1 · s-1. Our results indicate that although the reaction of ferric myeloperoxidase with O2- is an order of magnitude slower than with H2O2, the former reaction is sufficiently rapid to influence myeloperoxidase-dependent production of hypochlorous acid by stimulated neutrophils.",

keywords = "Hydrogen peroxide, Myeloperoxidase, Pulse radiolysis, Superoxide",

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T1 - A pulse radiolysis investigation of the reactions of myeloperoxidase with superoxide and hydrogen peroxide

AU - Kettle, Anthony J.

AU - Sangster, David F.

AU - Gebicki, Janusz M.

AU - Winterbourn, Christine C.

PY - 1988/8/31

Y1 - 1988/8/31

N2 - Using pulse radiolysis, the rate constant for the reaction of ferric myeloperoxidase with O2- to give compound III was measured at pH 7.8, and values of 2.1 · 106 M-1 · s-1 for equine ferric myeloperoxidase and 1.1 · 106 M-1 · s-1 for human ferric myeloperoxidase were obtained. Under the same conditions, the rate constant for the reaction of human ferric myeloperoxidase with H2O2 to give compound I was 3.1 · 107 M-1 · s-1. Our results indicate that although the reaction of ferric myeloperoxidase with O2- is an order of magnitude slower than with H2O2, the former reaction is sufficiently rapid to influence myeloperoxidase-dependent production of hypochlorous acid by stimulated neutrophils.

AB - Using pulse radiolysis, the rate constant for the reaction of ferric myeloperoxidase with O2- to give compound III was measured at pH 7.8, and values of 2.1 · 106 M-1 · s-1 for equine ferric myeloperoxidase and 1.1 · 106 M-1 · s-1 for human ferric myeloperoxidase were obtained. Under the same conditions, the rate constant for the reaction of human ferric myeloperoxidase with H2O2 to give compound I was 3.1 · 107 M-1 · s-1. Our results indicate that although the reaction of ferric myeloperoxidase with O2- is an order of magnitude slower than with H2O2, the former reaction is sufficiently rapid to influence myeloperoxidase-dependent production of hypochlorous acid by stimulated neutrophils.

KW - Hydrogen peroxide

KW - Myeloperoxidase

KW - Pulse radiolysis

KW - Superoxide

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JF - Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular

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A pulse radiolysis investigation of the reactions of myeloperoxidase with superoxide and hydrogen peroxide

Abstract

Keywords

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