A rapid co-culture stamping device for studying intercellular communication

Amin Hassanzadeh-Barforoushi, Jonathan Shemesh, Nona Farbehi, Mohsen Asadnia, Guan Heng Yeoh, Richard P. Harvey, Robert E. Nordon, Majid Ebrahimi Warkiani*

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Citations (Scopus)
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Abstract

Regulation of tissue development and repair depends on communication between neighbouring cells. Recent advances in cell micro-contact printing and microfluidics have facilitated the in-vitro study of homotypic and heterotypic cell-cell interaction. Nonetheless, these techniques are still complicated to perform and as a result, are seldom used by biologists. We report here development of a temporarily sealed microfluidic stamping device which utilizes a novel valve design for patterning two adherent cell lines with well-defined interlacing configurations to study cell-cell interactions. We demonstrate post-stamping cell viability of >95%, the stamping of multiple adherent cell types, and the ability to control the seeded cell density. We also show viability, proliferation and migration of cultured cells, enabling analysis of co-culture boundary conditions on cell fate. We also developed an in-vitro model of endothelial and cardiac stem cell interactions, which are thought to regulate coronary repair after myocardial injury. The stamp is fabricated using microfabrication techniques, is operated with a lab pipettor and uses very low reagent volumes of 20 μl with cell injection efficiency of >70%. This easy-to-use device provides a general strategy for micro-patterning of multiple cell types and will be important for studying cell-cell interactions in a multitude of applications.

Original languageEnglish
Article number35618
Pages (from-to)1-11
Number of pages11
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 18 Oct 2016

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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    Hassanzadeh-Barforoushi, A., Shemesh, J., Farbehi, N., Asadnia, M., Yeoh, G. H., Harvey, R. P., ... Warkiani, M. E. (2016). A rapid co-culture stamping device for studying intercellular communication. Scientific Reports, 6, 1-11. [35618]. https://doi.org/10.1038/srep35618