A rapid microfluidic stamping device for studying cardiac stem cells and endothelial cells co-culture

A. H. Barforoushi; J. Shemesh; N. Farbehi; M. Asadnia; G. H. Yeoh; R. E. Nordon; M. E. Warkiani

A rapid microfluidic stamping device for studying cardiac stem cells and endothelial cells co-culture

A. H. Barforoushi, J. Shemesh, N. Farbehi, M. Asadnia, G. H. Yeoh, R. E. Nordon, M. E. Warkiani^*

^*Corresponding author for this work

School of Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution

Abstract

Many biological processes in the body are regulated by synchronized activity between two cell types. Recent advances in cell μcontact printing have facilitated the in-vitro study of homotypic and heterotypic cell-cell interaction. However, these techniques are still complicated to perform and are seldom used by biologists. We report here development of a novel microfluidic stamping device for patterning two adherent cell lines with well-defined interlacing configurations to study cell-cell spatial interactions. To demonstrate the stamp's capabilities, we developed an in-vitro model of endothelial and cardiac mesenchymal stem cell interactions, which are thought to regulate coronary repair after myocardial injury.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	1069-1070
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016

Other

Other	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country	Ireland
City	Dublin
Period	9/10/16 → 13/10/16

Keywords

Co-culture
Collective cell migration
Microfluidics
Stem cells

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Barforoushi, A. H., Shemesh, J., Farbehi, N., Asadnia, M., Yeoh, G. H., Nordon, R. E., & Warkiani, M. E. (2016). A rapid microfluidic stamping device for studying cardiac stem cells and endothelial cells co-culture. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 1069-1070). Chemical and Biological Microsystems Society.

Barforoushi, A. H. ; Shemesh, J. ; Farbehi, N. ; Asadnia, M. ; Yeoh, G. H. ; Nordon, R. E. ; Warkiani, M. E. / A rapid microfluidic stamping device for studying cardiac stem cells and endothelial cells co-culture. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 1069-1070

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title = "A rapid microfluidic stamping device for studying cardiac stem cells and endothelial cells co-culture",

abstract = "Many biological processes in the body are regulated by synchronized activity between two cell types. Recent advances in cell μcontact printing have facilitated the in-vitro study of homotypic and heterotypic cell-cell interaction. However, these techniques are still complicated to perform and are seldom used by biologists. We report here development of a novel microfluidic stamping device for patterning two adherent cell lines with well-defined interlacing configurations to study cell-cell spatial interactions. To demonstrate the stamp's capabilities, we developed an in-vitro model of endothelial and cardiac mesenchymal stem cell interactions, which are thought to regulate coronary repair after myocardial injury.",

keywords = "Co-culture, Collective cell migration, Microfluidics, Stem cells",

author = "Barforoushi, {A. H.} and J. Shemesh and N. Farbehi and M. Asadnia and Yeoh, {G. H.} and Nordon, {R. E.} and Warkiani, {M. E.}",

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Barforoushi, AH, Shemesh, J, Farbehi, N, Asadnia, M, Yeoh, GH, Nordon, RE & Warkiani, ME 2016, A rapid microfluidic stamping device for studying cardiac stem cells and endothelial cells co-culture. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, pp. 1069-1070, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 9/10/16.

A rapid microfluidic stamping device for studying cardiac stem cells and endothelial cells co-culture. / Barforoushi, A. H.; Shemesh, J.; Farbehi, N.; Asadnia, M.; Yeoh, G. H.; Nordon, R. E.; Warkiani, M. E.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 1069-1070.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution

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T1 - A rapid microfluidic stamping device for studying cardiac stem cells and endothelial cells co-culture

AU - Barforoushi, A. H.

AU - Shemesh, J.

AU - Farbehi, N.

AU - Asadnia, M.

AU - Yeoh, G. H.

AU - Nordon, R. E.

AU - Warkiani, M. E.

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AB - Many biological processes in the body are regulated by synchronized activity between two cell types. Recent advances in cell μcontact printing have facilitated the in-vitro study of homotypic and heterotypic cell-cell interaction. However, these techniques are still complicated to perform and are seldom used by biologists. We report here development of a novel microfluidic stamping device for patterning two adherent cell lines with well-defined interlacing configurations to study cell-cell spatial interactions. To demonstrate the stamp's capabilities, we developed an in-vitro model of endothelial and cardiac mesenchymal stem cell interactions, which are thought to regulate coronary repair after myocardial injury.

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KW - Collective cell migration

KW - Microfluidics

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BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

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