A recyclable device for real-time cytokine monitoring in cell microenvironment

Guozhen Liu*, Chaomin Cao

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

Abstract

Monitoring cell-to-cell communication through cytokine secretions has enormous value in biology and medicine. Here, we demonstrate an amperometric sensor based on structure-switching aptamers for detection of cytokines in ex vivo. The hairpin aptamers, specific to Interferon-γ (IFN-γ) with intercalated redox probes are covalently attached to the graphene oxide layer to serve as bio-recognition moieties. In the presence of IFN-γ, the aptamers change conformation and release the trapped redox probes (Ru(NH3)63+) producing the electrochemical signal. This sensor is capable to quantitatively and dynamically detect IFN-γ secreted by immune cells into the cell culture medium with the sensitivity of 1.3 pg mL-1, and with no baseline drift even at high concentration of other nonspecific proteins.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages485-486
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2017
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 22 Oct 201726 Oct 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period22/10/1726/10/17

Keywords

  • Aptamers
  • Cytokines
  • IFN-γ
  • Real time sensing

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