A replication region of the IncHI plasmid, R27, is highly homologous with the RepFIA replicon of F

D. Saul, D. Lane, P. L. Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A region of the IncHl plasmid R27 has been found to share very close nucleotide sequence homology with the RepFIA replicon of F. This region has been located on a 1.6 kb segment of R27 plasmid DNA, and corresponds to ori‐2 and the E gene of F. The IncC repeat sequence region shows reduced homology, with the F repeats being an imperfect subset of a larger repeated sequence found in R27. The E gene homologue of R27 is able to initiate replication from the F ori‐2 sequence and to repress the E gene promoter of F. The results are consistent with the observed incompatibility behaviour of R27, and have a bearing on the specificity of interaction of E protein with its DNA‐binding sites.

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalMolecular Microbiology
Volume2
Issue number2
DOIs
Publication statusPublished - 1988
Externally publishedYes

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