A reversible fluorescence chemosensor for sequentially quantitative monitoring copper and sulfide in living cells

Qingtao Meng*, Run Zhang, Hongmin Jia, Xue Gao, Cuiping Wang, Yu Shi, Arun V. Everest-Dass, Zhiqiang Zhang

*Corresponding author for this work

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We report a novel, selective and sensitive strategy for the sequentially "ON-OFF-ON" fluorescent detection of Cu2+ and S2- based on a fluorescein derivative, FL. The specific binding of FL towards Cu2+ in aqueous and biological media led to the intensive green fluorescence quenching and a notable increase of the absorbance maximum at 480 nm. In the presence of S2-, the intensity and overall pattern of the fluorescence emission and UV-vis spectra of FL-Cu2+ ensemble were recovered since the abolishment of paramagnetic Cu2+. This displacement approach exhibited highly specificity, and sensitivity with detection limits of 3 nM for Cu2+ and 150 nM for S2-. The fluorescence "ON-OFF-ON" circle can be repeated to a minimum of 5 times by the alternative addition of Cu2+ and S2-, implying that FL is a renewable dual-functional chemosensor. The biocompatibility of FL toward breast carcinoma cells, MDA-MB-231 was confirmed by MTT assay. The reversible "ON-OFF-ON" fluorescent response of FL to Cu2+ and S2- in living system was further confirmed by confocal fluorescence imaging of living cells. The quantification of Cu2+ and S2- in single intact cell was realized by the flow cytometry analysis.

Original languageEnglish
Article number15572
Pages (from-to)294-301
Number of pages8
JournalTalanta
Volume143
DOIs
Publication statusPublished - 1 Oct 2015

Keywords

  • Fluorescein
  • Dual-functional chemosensor
  • Detection
  • Fluorescence imaging
  • Quantification

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