A ruthenium(ii) complex based turn-on electrochemiluminescence probe for the detection of nitric oxide

Wenzhu Zhang, Dan Zhao, Run Zhang, Zhiqiang Ye, Guilan Wang, Jingli Yuan*, Mei Yang

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    Electrochemiluminescence (ECL) detection technique using bipyridine-ruthenium(ii) complexes as probes is a highly sensitive and widely used method for the detection of various biological and bioactive molecules. In this work, the spectral, electrochemical and ECL properties of a chemically modified bipyridine-ruthenium(ii) complex, [Ru(bpy)2(dabpy)] 2+ (bpy: 2,2′-bipyridine; dabpy: 4-(3,4-diaminophenoxy)-2, 2′-bipyridine), were investigated and compared with those of its nitric oxide (NO)-reaction derivative [Ru(bpy)2(T-bpy)]2+ (T-bpy: 4-triazolephenoxy-2,2′-bipyridine) and [Ru(bpy)3] 2+. It was found that the ECL intensity of [Ru(bpy) 2(dabpy)]2+ could be selectively and sensitively enhanced by NO due to the formation of [Ru(bpy)2(T-bpy)]2+ in the presence of tri-n-propylamine. By using [Ru(bpy)2(dabpy)] 2+ as a probe, a sensitive and selective ECL method with a wide linear range (0.55 to 220.0 μM) and a low detection limit (0.28 μM) was established for the detection of NO in aqueous solutions and living cells. The results demonstrated the utility and advantages of the new ECL probe for the detection of NO in complicated biological samples.

    Original languageEnglish
    Pages (from-to)1867-1872
    Number of pages6
    JournalAnalyst
    Volume136
    Issue number9
    DOIs
    Publication statusPublished - 7 May 2011

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