A sensitive assay for creatine kinase in serum samples dried on paper: Enhanced thermal stability of the dried enzyme

Garth A. Nicholson; L. R. Griffiths

A sensitive assay for creatine kinase in serum samples dried on paper: Enhanced thermal stability of the dried enzyme

Garth A. Nicholson^*, L. R. Griffiths

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A new bioluminescent creatine kinase (CK) assay using purified luciferase was used to analyse CK activity in serum samples dried on filter paper. Enzyme activity was preserved for over 1 wk on paper stored at room temperature. At 60̊C, CK activity in liquid serum samples was rapidly inactivated, but the activity of enzyme stored on paper was preserved for at least 2 days.

Original language	English
Pages (from-to)	21-25
Number of pages	5
Journal	Pathology
Volume	15
Issue number	1
Publication status	Published - 1983
Externally published	Yes

Keywords

Luciferase
Paper adsorption
Serum creatine kinase
Thermal stability

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title = "A sensitive assay for creatine kinase in serum samples dried on paper: Enhanced thermal stability of the dried enzyme",

abstract = "A new bioluminescent creatine kinase (CK) assay using purified luciferase was used to analyse CK activity in serum samples dried on filter paper. Enzyme activity was preserved for over 1 wk on paper stored at room temperature. At 60̊C, CK activity in liquid serum samples was rapidly inactivated, but the activity of enzyme stored on paper was preserved for at least 2 days.",

keywords = "Luciferase, Paper adsorption, Serum creatine kinase, Thermal stability",

author = "Nicholson, {Garth A.} and Griffiths, {L. R.}",

year = "1983",

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pages = "21--25",

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T1 - A sensitive assay for creatine kinase in serum samples dried on paper

T2 - Enhanced thermal stability of the dried enzyme

AU - Nicholson, Garth A.

AU - Griffiths, L. R.

PY - 1983

Y1 - 1983

N2 - A new bioluminescent creatine kinase (CK) assay using purified luciferase was used to analyse CK activity in serum samples dried on filter paper. Enzyme activity was preserved for over 1 wk on paper stored at room temperature. At 60̊C, CK activity in liquid serum samples was rapidly inactivated, but the activity of enzyme stored on paper was preserved for at least 2 days.

AB - A new bioluminescent creatine kinase (CK) assay using purified luciferase was used to analyse CK activity in serum samples dried on filter paper. Enzyme activity was preserved for over 1 wk on paper stored at room temperature. At 60̊C, CK activity in liquid serum samples was rapidly inactivated, but the activity of enzyme stored on paper was preserved for at least 2 days.

KW - Luciferase

KW - Paper adsorption

KW - Serum creatine kinase

KW - Thermal stability

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M3 - Article

C2 - 6856339

AN - SCOPUS:0020687445

VL - 15

SP - 21

EP - 25

JO - Pathology

JF - Pathology

SN - 1465-3931

IS - 1

ER -