A sensitive assay for creatine kinase in serum samples dried on paper

Enhanced thermal stability of the dried enzyme

Garth A. Nicholson*, L. R. Griffiths

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new bioluminescent creatine kinase (CK) assay using purified luciferase was used to analyse CK activity in serum samples dried on filter paper. Enzyme activity was preserved for over 1 wk on paper stored at room temperature. At 60̊C, CK activity in liquid serum samples was rapidly inactivated, but the activity of enzyme stored on paper was preserved for at least 2 days.

Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalPathology
Volume15
Issue number1
Publication statusPublished - 1983
Externally publishedYes

Keywords

  • Luciferase
  • Paper adsorption
  • Serum creatine kinase
  • Thermal stability

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