A simple and rapid system for DNA and RNA isolation from diverse plants using handmade kit

Farhad Masoomi Aladizgeh, Leila Jabbari, Reza Khayam Nekouei, Ali Aalami

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Abstract

This protocol describes a rapid DNA and RNA extraction method for plant tissues. Hexadecyltrimethylammonium bromide (CTAB), sodium chloride (NaCl), tris base, and ethylenediaminetetraacetic acid (EDTA) are the main components of the extraction buffer. In contrast to all previously reported protocols, this extraction method does not require any stock solutions. This isolation buffer is potential of extracting both DNA and RNA simultaneously. Depending on the purpose of the project, the corresponding steps can be slightly altered to obtain either DNA or RNA. The big advantage of this method is to use general laboratory chemicals to make a powerful extraction buffer, resulting in high quality and quantity nucleic acid. Also, CTAB in this buffer is capable of isolating nucleic acid from recalcitrant plants enriched in secondary metabolites. Importantly, this method is recommended for the projects at which isolating nucleic acid in a short time is of crucial importance. This method probably is usable for all plant tissues and takes about an hour.
Original languageEnglish
Specialist publicationProtocol Exchange
DOIs
Publication statusPublished - 14 Mar 2016
Externally publishedYes

Bibliographical note

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