A single emm gene-specific oligonucleotide probe does not recognise all members of the Streptococcus pyogenes M type 1

Tim J. Penney, Diana R. Martin*, Liam C. Williams, Sheree A. de Malmanche, Peter L. Bergquist

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Serological typing of the streptococcal M protein has recently been challenged by a number of unique molecular methodologies based on oligonucleotide recognition of allelic variations within the M protein (emm) gene. In these methods, stringent hybridization of an oligonucleotide probe to a polymerase chain reaction amplified emm gene is used as confirmation of specific M type identity. A sample of 17 isolates from 7 previously defined distinct genotypes were tested using a single M1 oligonucleotide probe. Isolates from only three of the genotypes hybridized with the probe. The results demonstrate that a single emm-specific oligonucleotide probe can not identify all members of M type 1, as defined by conventional serotyping using polyclonal antisera.

Original languageEnglish
Pages (from-to)145-149
Number of pages5
JournalFEMS Microbiology Letters
Volume130
Issue number2-3
DOIs
Publication statusPublished - 1 Aug 1995
Externally publishedYes

Keywords

  • Allelic polymorphism
  • M protein
  • Molecular epidemiology
  • Oligonucleotide probe
  • Streptococcus pyogenes

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