A spectrophotometric method for the determination of lipid hydroperoxides

Mark Hicks; Janusz M. Gebicki

doi:10.1016/S0003-2697(79)80003-2

A spectrophotometric method for the determination of lipid hydroperoxides

Mark Hicks^*, Janusz M. Gebicki

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

155 Citations (Scopus)

Abstract

A modification of the iodine technique for peroxide determination is described, in which the reaction is carried out in deaerated methanol:acetic acid solution in a spectrophotometer cuvette. Measurements of the triiodide ions released are carried out either at 290 or 360 nm. In this solvent, the molar extinction coefficients are ε₂₉₀ = 4.41 × 10⁴ and ε₃₆₀ = 2.80 × 10⁴ liters mol^-1 cm^-1. Calibration with standard peroxide solutions showed that 1 mol of I₃^- is produced for every peroxide group added. In its present form, the method can be used to measure 1 nEq of lipid hydroperoxide.

Original language	English
Pages (from-to)	249-253
Number of pages	5
Journal	Analytical Biochemistry
Volume	99
Issue number	2
DOIs	https://doi.org/10.1016/S0003-2697(79)80003-2
Publication status	Published - 1 Nov 1979

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abstract = "A modification of the iodine technique for peroxide determination is described, in which the reaction is carried out in deaerated methanol:acetic acid solution in a spectrophotometer cuvette. Measurements of the triiodide ions released are carried out either at 290 or 360 nm. In this solvent, the molar extinction coefficients are ε290 = 4.41 × 104 and ε360 = 2.80 × 104 liters mol-1 cm-1. Calibration with standard peroxide solutions showed that 1 mol of I3- is produced for every peroxide group added. In its present form, the method can be used to measure 1 nEq of lipid hydroperoxide.",

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AU - Gebicki, Janusz M.

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N2 - A modification of the iodine technique for peroxide determination is described, in which the reaction is carried out in deaerated methanol:acetic acid solution in a spectrophotometer cuvette. Measurements of the triiodide ions released are carried out either at 290 or 360 nm. In this solvent, the molar extinction coefficients are ε290 = 4.41 × 104 and ε360 = 2.80 × 104 liters mol-1 cm-1. Calibration with standard peroxide solutions showed that 1 mol of I3- is produced for every peroxide group added. In its present form, the method can be used to measure 1 nEq of lipid hydroperoxide.

AB - A modification of the iodine technique for peroxide determination is described, in which the reaction is carried out in deaerated methanol:acetic acid solution in a spectrophotometer cuvette. Measurements of the triiodide ions released are carried out either at 290 or 360 nm. In this solvent, the molar extinction coefficients are ε290 = 4.41 × 104 and ε360 = 2.80 × 104 liters mol-1 cm-1. Calibration with standard peroxide solutions showed that 1 mol of I3- is produced for every peroxide group added. In its present form, the method can be used to measure 1 nEq of lipid hydroperoxide.

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U2 - 10.1016/S0003-2697(79)80003-2

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

A spectrophotometric method for the determination of lipid hydroperoxides

Abstract

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