A study on the effect of the internal exposure to 210Po on the excretion of urinary proteins in rats

Baki Sadi, Chunsheng Li, Raymond Ko, Joseph Daka, Hamdi Yusuf, Heather Wyatt, Joel Surette, Nick Priest, Nobuyuki Hamada

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1 Citation (Scopus)

Abstract

This study was designed to assess the feasibility of a noninvasive urine specimen for the detection of proteins as indicators of internal exposure to ionizing radiation. Three groups of rats (five in each group) were intravenously injected with 1601 ± 376, 10,846 ± 591 and 48,467 ± 2812 Bq of 210Po in citrate form. A sham-exposed control group of five rats was intravenously injected with sterile physiological saline. Daily urine samples were collected over 4 days following injection. Purification and pre-concentration of urinary proteins were carried out by ultrafiltration using a 3000 Da molecular weight cutoff membrane filter. The concentration of common urinary proteins, namely albumin, alpha-1-acid glycoprotein, immunoglobulins IgA and IgG, was measured by an enzyme-linked immunosorbent assay. Urinary excretion of albumin decreased dose-dependently (p < 0.05) 96 h post-injection relative to the control group. In contrast, no statistically significant effects were observed for other proteins tested. The dose-dependent decrease in urinary excretion of albumin observed in this study underscores the need for further research, which may lead to the discovery of new biomarkers that would reflect the changes in the primary target organs for deposition of 210Po.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalRadiation and Environmental Biophysics
Volume55
Issue number2
DOIs
Publication statusPublished - May 2016
Externally publishedYes

Keywords

  • urinary proteins
  • biomarkers
  • internal exposure
  • polonium-210

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