A targeted proteomic strategy for the measurement of oral cancer candidate biomarkers in human saliva

Rebeca Kawahara, James G. Bollinger, César Rivera, Ana Carolina P. Ribeiro, Thaís Bianca Brandão, Adriana F. Paes Leme, Michael J. MacCoss

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Head and neck cancers, including oral squamous cell carcinoma (OSCC), are the sixth most common malignancy in the world and are characterized by poor prognosis and a low survival rate. Saliva is oral fluid with intimate contact with OSCC. Besides non‐invasive, simple, and rapid to collect, saliva is a potential source of biomarkers. In this study, we build an SRM assay that targets fourteen OSCC candidate biomarker proteins, which were evaluated in a set of clinically‐derived saliva samples. Using Skyline software package, we demonstrated a statistically significant higher abundance of the C1R, LCN2, SLPI, FAM49B, TAGLN2, CFB, C3, C4B, LRG1, SERPINA1 candidate biomarkers in the saliva of OSCC patients. Furthermore, our study also demonstrated that CFB, C3, C4B, SERPINA1 and LRG1 are associated with the risk of developing OSCC. Overall, this study successfully used targeted proteomics to measure in saliva a panel of biomarker candidates for OSCC.
Original languageEnglish
Pages (from-to)159-173
Number of pages15
JournalProteomics
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 2016
Externally publishedYes

Keywords

  • Biomedicine
  • Oral cancer
  • Saliva
  • Selected reaction monitoring
  • Skyline

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