A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease

Emily K. Don; Isabel Formella; Andrew P. Badrock; Thomas E. Hall; Marco Morsch; Elinor Hortle; Alison Hogan; Sharron Chow; Serene S. L. Gwee; Jack J. Stoddart; Garth Nicholson; Roger Chung; Nicholas J. Cole

doi:10.1089/zeb.2016.1321

A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease

Emily K. Don^*, Isabel Formella, Andrew P. Badrock, Thomas E. Hall, Marco Morsch, Elinor Hortle, Alison Hogan, Sharron Chow, Serene S. L. Gwee, Jack J. Stoddart, Garth Nicholson, Roger Chung, Nicholas J. Cole

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover effective therapeutics. We have generated a Tol2 Gateway-compatible toolbox to study neurodegenerative disorders in zebrafish, which includes promoters for astrocytes, microglia and motor neurons, multiple fluorophores, and compatibility for the introduction of genes of interest or disease-linked genes. This toolbox will advance the rapid and flexible generation of zebrafish models to discover the biology of the nervous system and the disease processes that lead to neurodegeneration.

Original language	English
Pages (from-to)	69-72
Number of pages	4
Journal	Zebrafish
Volume	14
Issue number	1
Early online date	15 Sept 2016
DOIs	https://doi.org/10.1089/zeb.2016.1321
Publication status	Published - 1 Feb 2017

Keywords

disease model
neurodegenerative disease
Tol2 gateway
transgenesis

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10.1089/zeb.2016.1321

Cite this

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title = "A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease",

abstract = "Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover effective therapeutics. We have generated a Tol2 Gateway-compatible toolbox to study neurodegenerative disorders in zebrafish, which includes promoters for astrocytes, microglia and motor neurons, multiple fluorophores, and compatibility for the introduction of genes of interest or disease-linked genes. This toolbox will advance the rapid and flexible generation of zebrafish models to discover the biology of the nervous system and the disease processes that lead to neurodegeneration.",

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AU - Don, Emily K.

AU - Formella, Isabel

AU - Badrock, Andrew P.

AU - Hall, Thomas E.

AU - Morsch, Marco

AU - Hortle, Elinor

AU - Hogan, Alison

AU - Chow, Sharron

AU - Gwee, Serene S. L.

AU - Stoddart, Jack J.

AU - Nicholson, Garth

AU - Chung, Roger

AU - Cole, Nicholas J.

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AB - Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover effective therapeutics. We have generated a Tol2 Gateway-compatible toolbox to study neurodegenerative disorders in zebrafish, which includes promoters for astrocytes, microglia and motor neurons, multiple fluorophores, and compatibility for the introduction of genes of interest or disease-linked genes. This toolbox will advance the rapid and flexible generation of zebrafish models to discover the biology of the nervous system and the disease processes that lead to neurodegeneration.

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KW - transgenesis

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A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease

Abstract

Keywords

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Investigating the pathogenesis of motor neuron disease using zebrafish models

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A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease

Abstract

Keywords

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Investigating the pathogenesis of motor neuron disease using zebrafish models

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