A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei

Merja Penttilä*, Helena Nevalainen, Marjaana Rättö, Elina Salminen, Jonathan Knowles

*Corresponding author for this work

Research output: Contribution to journalArticle

471 Citations (Scopus)

Abstract

An efficient transformation system for the cellulolytic filamentous fungus Trichoderma reesei has been developed. Transformation was obtained with plasmid carrying the dominant selectable marker amdS or the argB gene of Aspergillus nidulans, which was found to complement the respective argB mutation of T. reesei. The transformation frequency can be up to 600 transformants per μg of transforming DNA. The efficiency of co-transformation with unselected DNA was high (approx. 80%). The transforming DNA was found to be integrated at several different locations, often in multiple tandem copies in the T. reesei genome. In addition, the Escherichia coli β-galactosidase was expressed in T. reesei in enzymatically active form from the A. nidulans gpd promoter.

Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalGene
Volume61
Issue number2
DOIs
Publication statusPublished - 1987
Externally publishedYes

Keywords

  • cotransformation
  • heterologous expression
  • integration
  • lignocellulose
  • Recombinant DNA

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