A whole-cell bioreporter approach for the genotoxicity assessment of bioavailability of toxic compounds in contaminated soil in China

Yizhi Song, Bo Jiang, Sicong Tian, Hui Tang, Zengjun Liu, Chuan Li, Jianli Jia, Wei E Huang, Xu Zhang, Guanghe Li*

*Corresponding author for this work

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A whole-cell bacterial bioreporter Acinetobacter baylyi strain ADP1_recA_lux that responds to genotoxins was employed to directly assess the adverse effects of the bioavailable fraction of mitomycin C (MMC), benzo[a]pyrene (BaP), chromium (VI) and lead (II) in amended soils and soil samples from two fragile areas in China without soil pre-treatment. The amended soils containing pollutants with the concentrations as low as 0.4 mg/kg MMC, 0.5 mg/kg BaP, 520 mg/kg Cr (VI) and 2072 mg/kg Pb (II) were found to be toxic. Soil particle-associated pollutants accounted for 86%, 100%, 29%, and 92% of the genotoxicity in the MMC, BaP, Cr (VI), and Pb (II) amended soil, respectively. The soils from contaminated sites were also valid to be genotoxic. The results suggest both free and soil particle-associated pollutants are bioavailable to soil organisms and a solid-phase contact bioreporter assay to soil contamination could provide a rapid screening tool for environmental risk assessment.

Original languageEnglish
Pages (from-to)178-184
Number of pages7
JournalEnvironmental Pollution
Volume195
DOIs
Publication statusPublished - Dec 2014
Externally publishedYes

Keywords

  • Whole-cell bioreporter
  • Soil
  • Genotoxicity
  • Bioavailability
  • PAHs
  • BACTERIAL BIOREPORTER
  • NITRIFYING BACTERIA
  • BIOREMEDIATION
  • BIOSENSOR
  • CADMIUM
  • CONSTRUCTION
  • ENVIRONMENT
  • REPORTER
  • DRUGS
  • SENSORS

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