AadT, a new weapon in Acinetobacter's fight against antibiotics

Varsha Naidu; Bhumika Shah; Claire Maher; Ian T. Paulsen; Karl A. Hassan

doi:10.1099/mic.0.001341

AadT, a new weapon in Acinetobacter's fight against antibiotics

Varsha Naidu, Bhumika Shah, Claire Maher, Ian T. Paulsen, Karl A. Hassan^*

^*Corresponding author for this work

School of Natural Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

59 Downloads (Pure)

Abstract

Genes encoding a novel multidrug efflux pump, AadT, from the Drug:H⁺ antiporter 2 family, were discovered in Acinetobacter multidrug resistance plasmids. Here, we profiled the antimicrobial resistance potential, and examined the distribution of these genes. aadT homologs were found in many Acinetobacter and other Gram-negative species and were typically adjacent to novel variants of adeAB(C), which encodes a major tripartite efflux pump in Acinetobacter. The AadT pump decreased bacterial susceptibility to at least eight diverse antimicrobials, including antibiotics (erythromycin and tetracycline), biocides (chlorhexidine), and dyes (ethidium bromide and DAPI) and was able to mediate ethidium transport. These results show that AadT is a multidrug efflux pump in the Acinetobacter resistance arsenal and may cooperate with variants of AdeAB(C).

Original language	English
Article number	001341
Pages (from-to)	1-6
Number of pages	6
Journal	Microbiology
Volume	169
Issue number	5
Early online date	30 May 2023
DOIs	https://doi.org/10.1099/mic.0.001341
Publication status	Published - 2023

Bibliographical note

Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Acinetobacter baumannii
Acinetobacter plasmids
Antimicrobial Resistance
efflux pumps
Major Facilitator Superfamily (MFS)
RND efflux

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10.1099/mic.0.001341

Publisher version (open access)Final published version, 1.35 MBLicence: CC BY

Cite this

@article{9753607f98184a1cbbefb4f271f002b7,

title = "AadT, a new weapon in Acinetobacter's fight against antibiotics",

abstract = "Genes encoding a novel multidrug efflux pump, AadT, from the Drug:H+ antiporter 2 family, were discovered in Acinetobacter multidrug resistance plasmids. Here, we profiled the antimicrobial resistance potential, and examined the distribution of these genes. aadT homologs were found in many Acinetobacter and other Gram-negative species and were typically adjacent to novel variants of adeAB(C), which encodes a major tripartite efflux pump in Acinetobacter. The AadT pump decreased bacterial susceptibility to at least eight diverse antimicrobials, including antibiotics (erythromycin and tetracycline), biocides (chlorhexidine), and dyes (ethidium bromide and DAPI) and was able to mediate ethidium transport. These results show that AadT is a multidrug efflux pump in the Acinetobacter resistance arsenal and may cooperate with variants of AdeAB(C).",

keywords = "Acinetobacter baumannii, Acinetobacter plasmids, Antimicrobial Resistance, efflux pumps, Major Facilitator Superfamily (MFS), RND efflux",

author = "Varsha Naidu and Bhumika Shah and Claire Maher and Paulsen, {Ian T.} and Hassan, {Karl A.}",

note = "Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. ",

year = "2023",

doi = "10.1099/mic.0.001341",

language = "English",

volume = "169",

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AU - Naidu, Varsha

AU - Shah, Bhumika

AU - Maher, Claire

AU - Paulsen, Ian T.

AU - Hassan, Karl A.

N1 - Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2023

Y1 - 2023

N2 - Genes encoding a novel multidrug efflux pump, AadT, from the Drug:H+ antiporter 2 family, were discovered in Acinetobacter multidrug resistance plasmids. Here, we profiled the antimicrobial resistance potential, and examined the distribution of these genes. aadT homologs were found in many Acinetobacter and other Gram-negative species and were typically adjacent to novel variants of adeAB(C), which encodes a major tripartite efflux pump in Acinetobacter. The AadT pump decreased bacterial susceptibility to at least eight diverse antimicrobials, including antibiotics (erythromycin and tetracycline), biocides (chlorhexidine), and dyes (ethidium bromide and DAPI) and was able to mediate ethidium transport. These results show that AadT is a multidrug efflux pump in the Acinetobacter resistance arsenal and may cooperate with variants of AdeAB(C).

AB - Genes encoding a novel multidrug efflux pump, AadT, from the Drug:H+ antiporter 2 family, were discovered in Acinetobacter multidrug resistance plasmids. Here, we profiled the antimicrobial resistance potential, and examined the distribution of these genes. aadT homologs were found in many Acinetobacter and other Gram-negative species and were typically adjacent to novel variants of adeAB(C), which encodes a major tripartite efflux pump in Acinetobacter. The AadT pump decreased bacterial susceptibility to at least eight diverse antimicrobials, including antibiotics (erythromycin and tetracycline), biocides (chlorhexidine), and dyes (ethidium bromide and DAPI) and was able to mediate ethidium transport. These results show that AadT is a multidrug efflux pump in the Acinetobacter resistance arsenal and may cooperate with variants of AdeAB(C).

KW - Acinetobacter baumannii

KW - Acinetobacter plasmids

KW - Antimicrobial Resistance

KW - efflux pumps

KW - Major Facilitator Superfamily (MFS)

KW - RND efflux

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UR - http://purl.org/au-research/grants/arc/FT180100123

UR - http://purl.org/au-research/grants/nhmrc/1120298

U2 - 10.1099/mic.0.001341

DO - 10.1099/mic.0.001341

M3 - Article

C2 - 37252766

SN - 1350-0872

VL - 169

SP - 1

EP - 6

JO - Microbiology

JF - Microbiology

IS - 5

M1 - 001341

ER -

AadT, a new weapon in Acinetobacter's fight against antibiotics

Abstract

Bibliographical note

Keywords

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Circuit Breaker: Investigating the regulatory circuits controlling expression of drug efflux pumps in the nosocomial pathogen Acinetobacter baumannii

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AadT, a new weapon in Acinetobacter's fight against antibiotics

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

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Circuit Breaker: Investigating the regulatory circuits controlling expression of drug efflux pumps in the nosocomial pathogen Acinetobacter baumannii

Cite this