Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags

Yuling Wang*, Eugene J H Wee, Matt Trau

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Sensitive and accurate total genomic DNA methylation analysis was demonstrated by surface-enhanced Raman scattering (SERS) via embedded internal SERS nanotags. The assay was sensitive to 0.2 ng input DNA while differentiating as low as 6.25% changes in DNA methylation. The method could also successfully differentiate cells before and after de-methylating drug-treatment and between tumor and normal biopsies.

Original languageEnglish
Pages (from-to)3560-3563
Number of pages4
JournalChemical Communications
Volume52
Issue number17
DOIs
Publication statusPublished - 28 Feb 2016
Externally publishedYes

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