Research output per year
Research output per year
Anna Guller, Inna Trusova, Elena Petersen, Anatoly Shekhter, Alexander Kurkov, Yi Qian, Andrei Zvyagin
Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review
Rationale: Tissue engineering (TE) is an emerging alternative approach to create models of human malignant tumors for experimental oncology, personalized medicine and drug discovery studies. Being the bottom-up strategy, TE provides an opportunity to control and explore the role of every component of the model system, including cellular populations, supportive scaffolds and signalling molecules. Objectives: As an initial step to create a new ex vivo TE model of cancer, we optimized protocols to obtain organ-specific acellular matrices and evaluated their potential as TE scaffolds for culture of normal and tumor cells. Methods and results: Effective decellularization of animals'™ kidneys, ureter, lungs, heart, and liver has been achieved by detergent-based processing. The obtained scaffolds demonstrated biocompatibility and growthsupporting potential in combination with normal (Vero, MDCK) and tumor cell lines (C26, B16). Acellular scaffolds and TE constructs have been characterized and compared with morphological methods. Conclusions: The proposed methodology allows creation of sustainable 3D tumor TE constructs to explore the role of organ-specific cell-matrix interaction in tumorigenesis.
Original language | English |
---|---|
Title of host publication | Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems |
Editors | Benjamin J. Eggleton, Stefano Palomba |
Place of Publication | Bellingham, WA |
Publisher | SPIE |
Pages | 1-9 |
Number of pages | 9 |
Volume | 9668 |
ISBN (Print) | 9781628418903 |
DOIs | |
Publication status | Published - 2015 |
Event | SPIE Micro+Nano Materials, Devices, and Applications Symposium - Sydney, Australia Duration: 6 Dec 2015 → 9 Dec 2015 |
Name | SPIE - International Society for Optical Engineering Proceedings |
---|---|
Publisher | SPIE - International Society for Optical Engineering |
ISSN (Print) | 0277-786X |
Other | SPIE Micro+Nano Materials, Devices, and Applications Symposium |
---|---|
Country/Territory | Australia |
City | Sydney |
Period | 6/12/15 → 9/12/15 |
Research output: Contribution to journal › Review article › peer-review