TY - GEN
T1 - Acellular organ scaffolds for tumor tissue engineering
AU - Guller, Anna
AU - Trusova, Inna
AU - Petersen, Elena
AU - Shekhter, Anatoly
AU - Kurkov, Alexander
AU - Qian, Yi
AU - Zvyagin, Andrei
PY - 2015
Y1 - 2015
N2 - Rationale: Tissue engineering (TE) is an emerging alternative approach to create models of human malignant tumors for experimental oncology, personalized medicine and drug discovery studies. Being the bottom-up strategy, TE provides an opportunity to control and explore the role of every component of the model system, including cellular populations, supportive scaffolds and signalling molecules. Objectives: As an initial step to create a new ex vivo TE model of cancer, we optimized protocols to obtain organ-specific acellular matrices and evaluated their potential as TE scaffolds for culture of normal and tumor cells. Methods and results: Effective decellularization of animals'™ kidneys, ureter, lungs, heart, and liver has been achieved by detergent-based processing. The obtained scaffolds demonstrated biocompatibility and growthsupporting potential in combination with normal (Vero, MDCK) and tumor cell lines (C26, B16). Acellular scaffolds and TE constructs have been characterized and compared with morphological methods. Conclusions: The proposed methodology allows creation of sustainable 3D tumor TE constructs to explore the role of organ-specific cell-matrix interaction in tumorigenesis.
AB - Rationale: Tissue engineering (TE) is an emerging alternative approach to create models of human malignant tumors for experimental oncology, personalized medicine and drug discovery studies. Being the bottom-up strategy, TE provides an opportunity to control and explore the role of every component of the model system, including cellular populations, supportive scaffolds and signalling molecules. Objectives: As an initial step to create a new ex vivo TE model of cancer, we optimized protocols to obtain organ-specific acellular matrices and evaluated their potential as TE scaffolds for culture of normal and tumor cells. Methods and results: Effective decellularization of animals'™ kidneys, ureter, lungs, heart, and liver has been achieved by detergent-based processing. The obtained scaffolds demonstrated biocompatibility and growthsupporting potential in combination with normal (Vero, MDCK) and tumor cell lines (C26, B16). Acellular scaffolds and TE constructs have been characterized and compared with morphological methods. Conclusions: The proposed methodology allows creation of sustainable 3D tumor TE constructs to explore the role of organ-specific cell-matrix interaction in tumorigenesis.
UR - http://www.scopus.com/inward/record.url?scp=84959874504&partnerID=8YFLogxK
U2 - 10.1117/12.2202473
DO - 10.1117/12.2202473
M3 - Conference proceeding contribution
AN - SCOPUS:84959874504
SN - 9781628418903
VL - 9668
T3 - SPIE - International Society for Optical Engineering Proceedings
SP - 1
EP - 9
BT - Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems
A2 - Eggleton, Benjamin J.
A2 - Palomba, Stefano
PB - SPIE
CY - Bellingham, WA
T2 - SPIE Micro+Nano Materials, Devices, and Applications Symposium
Y2 - 6 December 2015 through 9 December 2015
ER -