Acellular organ scaffolds for tumor tissue engineering

Anna Guller; Inna Trusova; Elena Petersen; Anatoly Shekhter; Alexander Kurkov; Yi Qian; Andrei Zvyagin

doi:10.1117/12.2202473

Acellular organ scaffolds for tumor tissue engineering

Anna Guller, Inna Trusova, Elena Petersen, Anatoly Shekhter, Alexander Kurkov, Yi Qian, Andrei Zvyagin

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

4 Citations (Scopus)

218 Downloads (Pure)

Abstract

Rationale: Tissue engineering (TE) is an emerging alternative approach to create models of human malignant tumors for experimental oncology, personalized medicine and drug discovery studies. Being the bottom-up strategy, TE provides an opportunity to control and explore the role of every component of the model system, including cellular populations, supportive scaffolds and signalling molecules. Objectives: As an initial step to create a new ex vivo TE model of cancer, we optimized protocols to obtain organ-specific acellular matrices and evaluated their potential as TE scaffolds for culture of normal and tumor cells. Methods and results: Effective decellularization of animals'™ kidneys, ureter, lungs, heart, and liver has been achieved by detergent-based processing. The obtained scaffolds demonstrated biocompatibility and growthsupporting potential in combination with normal (Vero, MDCK) and tumor cell lines (C26, B16). Acellular scaffolds and TE constructs have been characterized and compared with morphological methods. Conclusions: The proposed methodology allows creation of sustainable 3D tumor TE constructs to explore the role of organ-specific cell-matrix interaction in tumorigenesis.

Original language	English
Title of host publication	Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems
Editors	Benjamin J. Eggleton, Stefano Palomba
Place of Publication	Bellingham, WA
Publisher	SPIE
Pages	1-9
Number of pages	9
Volume	9668
ISBN (Print)	9781628418903
DOIs	https://doi.org/10.1117/12.2202473
Publication status	Published - 2015
Event	SPIE Micro+Nano Materials, Devices, and Applications Symposium - Sydney, Australia Duration: 6 Dec 2015 → 9 Dec 2015

Publication series

Name	SPIE - International Society for Optical Engineering Proceedings
Publisher	SPIE - International Society for Optical Engineering
ISSN (Print)	0277-786X

Other

Other	SPIE Micro+Nano Materials, Devices, and Applications Symposium
Country/Territory	Australia
City	Sydney
Period	6/12/15 → 9/12/15

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10.1117/12.2202473

Publisher version (open access)Final published version, 781 KB

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Guller, A., Trusova, I., Petersen, E., Shekhter, A., Kurkov, A., Qian, Y., & Zvyagin, A. (2015). Acellular organ scaffolds for tumor tissue engineering. In B. J. Eggleton, & S. Palomba (Eds.), Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems (Vol. 9668, pp. 1-9). Article 96684G (SPIE - International Society for Optical Engineering Proceedings). SPIE. https://doi.org/10.1117/12.2202473

@inproceedings{230adc4f2a034c3e892f24c88a5ae4cd,

title = "Acellular organ scaffolds for tumor tissue engineering",

abstract = "Rationale: Tissue engineering (TE) is an emerging alternative approach to create models of human malignant tumors for experimental oncology, personalized medicine and drug discovery studies. Being the bottom-up strategy, TE provides an opportunity to control and explore the role of every component of the model system, including cellular populations, supportive scaffolds and signalling molecules. Objectives: As an initial step to create a new ex vivo TE model of cancer, we optimized protocols to obtain organ-specific acellular matrices and evaluated their potential as TE scaffolds for culture of normal and tumor cells. Methods and results: Effective decellularization of animals'{\texttrademark} kidneys, ureter, lungs, heart, and liver has been achieved by detergent-based processing. The obtained scaffolds demonstrated biocompatibility and growthsupporting potential in combination with normal (Vero, MDCK) and tumor cell lines (C26, B16). Acellular scaffolds and TE constructs have been characterized and compared with morphological methods. Conclusions: The proposed methodology allows creation of sustainable 3D tumor TE constructs to explore the role of organ-specific cell-matrix interaction in tumorigenesis.",

author = "Anna Guller and Inna Trusova and Elena Petersen and Anatoly Shekhter and Alexander Kurkov and Yi Qian and Andrei Zvyagin",

year = "2015",

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language = "English",

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volume = "9668",

series = "SPIE - International Society for Optical Engineering Proceedings",

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booktitle = "Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems",

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note = "SPIE Micro+Nano Materials, Devices, and Applications Symposium ; Conference date: 06-12-2015 Through 09-12-2015",

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Guller, A, Trusova, I, Petersen, E, Shekhter, A, Kurkov, A, Qian, Y & Zvyagin, A 2015, Acellular organ scaffolds for tumor tissue engineering. in BJ Eggleton & S Palomba (eds), Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems. vol. 9668, 96684G, SPIE - International Society for Optical Engineering Proceedings, SPIE, Bellingham, WA, pp. 1-9, SPIE Micro+Nano Materials, Devices, and Applications Symposium, Sydney, Australia, 6/12/15. https://doi.org/10.1117/12.2202473

Acellular organ scaffolds for tumor tissue engineering. / Guller, Anna; Trusova, Inna; Petersen, Elena et al.
Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems. ed. / Benjamin J. Eggleton; Stefano Palomba. Vol. 9668 Bellingham, WA: SPIE, 2015. p. 1-9 96684G (SPIE - International Society for Optical Engineering Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - Acellular organ scaffolds for tumor tissue engineering

AU - Guller, Anna

AU - Trusova, Inna

AU - Petersen, Elena

AU - Shekhter, Anatoly

AU - Kurkov, Alexander

AU - Qian, Yi

AU - Zvyagin, Andrei

PY - 2015

Y1 - 2015

N2 - Rationale: Tissue engineering (TE) is an emerging alternative approach to create models of human malignant tumors for experimental oncology, personalized medicine and drug discovery studies. Being the bottom-up strategy, TE provides an opportunity to control and explore the role of every component of the model system, including cellular populations, supportive scaffolds and signalling molecules. Objectives: As an initial step to create a new ex vivo TE model of cancer, we optimized protocols to obtain organ-specific acellular matrices and evaluated their potential as TE scaffolds for culture of normal and tumor cells. Methods and results: Effective decellularization of animals'™ kidneys, ureter, lungs, heart, and liver has been achieved by detergent-based processing. The obtained scaffolds demonstrated biocompatibility and growthsupporting potential in combination with normal (Vero, MDCK) and tumor cell lines (C26, B16). Acellular scaffolds and TE constructs have been characterized and compared with morphological methods. Conclusions: The proposed methodology allows creation of sustainable 3D tumor TE constructs to explore the role of organ-specific cell-matrix interaction in tumorigenesis.

AB - Rationale: Tissue engineering (TE) is an emerging alternative approach to create models of human malignant tumors for experimental oncology, personalized medicine and drug discovery studies. Being the bottom-up strategy, TE provides an opportunity to control and explore the role of every component of the model system, including cellular populations, supportive scaffolds and signalling molecules. Objectives: As an initial step to create a new ex vivo TE model of cancer, we optimized protocols to obtain organ-specific acellular matrices and evaluated their potential as TE scaffolds for culture of normal and tumor cells. Methods and results: Effective decellularization of animals'™ kidneys, ureter, lungs, heart, and liver has been achieved by detergent-based processing. The obtained scaffolds demonstrated biocompatibility and growthsupporting potential in combination with normal (Vero, MDCK) and tumor cell lines (C26, B16). Acellular scaffolds and TE constructs have been characterized and compared with morphological methods. Conclusions: The proposed methodology allows creation of sustainable 3D tumor TE constructs to explore the role of organ-specific cell-matrix interaction in tumorigenesis.

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M3 - Conference proceeding contribution

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VL - 9668

T3 - SPIE - International Society for Optical Engineering Proceedings

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BT - Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems

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A2 - Palomba, Stefano

PB - SPIE

CY - Bellingham, WA

T2 - SPIE Micro+Nano Materials, Devices, and Applications Symposium

Y2 - 6 December 2015 through 9 December 2015

ER -

Acellular organ scaffolds for tumor tissue engineering

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Combination drug therapy of glioblastoma: lessons from 3D in vitro models and the roadmap for future research

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