Acellular organ scaffolds for tumor tissue engineering

Anna Guller, Inna Trusova, Elena Petersen, Anatoly Shekhter, Alexander Kurkov, Yi Qian, Andrei Zvyagin

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

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Abstract

Rationale: Tissue engineering (TE) is an emerging alternative approach to create models of human malignant tumors for experimental oncology, personalized medicine and drug discovery studies. Being the bottom-up strategy, TE provides an opportunity to control and explore the role of every component of the model system, including cellular populations, supportive scaffolds and signalling molecules. Objectives: As an initial step to create a new ex vivo TE model of cancer, we optimized protocols to obtain organ-specific acellular matrices and evaluated their potential as TE scaffolds for culture of normal and tumor cells. Methods and results: Effective decellularization of animals'™ kidneys, ureter, lungs, heart, and liver has been achieved by detergent-based processing. The obtained scaffolds demonstrated biocompatibility and growthsupporting potential in combination with normal (Vero, MDCK) and tumor cell lines (C26, B16). Acellular scaffolds and TE constructs have been characterized and compared with morphological methods. Conclusions: The proposed methodology allows creation of sustainable 3D tumor TE constructs to explore the role of organ-specific cell-matrix interaction in tumorigenesis.

Original languageEnglish
Title of host publicationProc. SPIE 9668, Micro+Nano Materials, Devices, and Systems
EditorsBenjamin J. Eggleton, Stefano Palomba
Place of PublicationBellingham, WA
PublisherSPIE
Pages1-9
Number of pages9
Volume9668
ISBN (Print)9781628418903
DOIs
Publication statusPublished - 2015
EventSPIE Micro+Nano Materials, Devices, and Applications Symposium - Sydney, Australia
Duration: 6 Dec 20159 Dec 2015

Publication series

NameSPIE - International Society for Optical Engineering Proceedings
PublisherSPIE - International Society for Optical Engineering
ISSN (Print)0277-786X

Other

OtherSPIE Micro+Nano Materials, Devices, and Applications Symposium
CountryAustralia
CitySydney
Period6/12/159/12/15

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Guller, A., Trusova, I., Petersen, E., Shekhter, A., Kurkov, A., Qian, Y., & Zvyagin, A. (2015). Acellular organ scaffolds for tumor tissue engineering. In B. J. Eggleton, & S. Palomba (Eds.), Proc. SPIE 9668, Micro+Nano Materials, Devices, and Systems (Vol. 9668, pp. 1-9). [96684G] (SPIE - International Society for Optical Engineering Proceedings). Bellingham, WA: SPIE. https://doi.org/10.1117/12.2202473