Activation of the mammalian cells by using light-sensitive ion channels

Mandy Siu Yu Lung, Paul Pilowsky, Ewa M. Goldys*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

With advances in molecular biology and gene cloning techniques, it is now possible to selectively stimulate living cells of interest by using an external light source. This is done by transfecting the cells of interest with a plasmid carrying the channelrhodopsin (ChR2) gene. By stimulating these transfected cells with laser, the light-sensitive ion channels ChR2 are opened, followed by an influx of cation resulting in cell activation. This combination of optical and genetic technique is known in the literature as optogenetics. It is particularly useful in the functional studies of excitable cells, such as neurons, muscle and endocrine cells, to mimic the stimulation from action potentials to trigger the release neurotransmitters and hormones. Here, we describe the methods needed to make selected mammalian cells (PC12) respond to light excitation.

Original languageEnglish
Title of host publicationSpectroscopic Methods of Analysis
Subtitle of host publicationMethods and Protocols
EditorsWlodek M. Bujalowski
Place of PublicationNew York
PublisherHumana Press Inc.
Pages241-251
Number of pages11
ISBN (Electronic)9781617798061
ISBN (Print)9781617798054
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume875
ISSN (Print)1064-3745

Fingerprint

Dive into the research topics of 'Activation of the mammalian cells by using light-sensitive ion channels'. Together they form a unique fingerprint.

Cite this