Activity-based identification of secreted serine proteases of the filamentous fungus, Ophiostoma

Caiyan Wu, Qiang Xu, Fei Liu*, K. M Helena Nevalainen

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A general activity probe was synthesized and applied to the supernatant of a filamentous fungus, Ophiostoma, culture to identify directly the secreted serine proteases by covalent enzyme labeling. The activity probe contained a chemically reactive group that reacted with, and thus covalently labeled, the serine residues of only active proteases and not heat-inactivated proteases. The activity probe also contained a fluorescent group that allowed for the subsequent visualization of the captured proteases in 1-D gels and their identification by N-terminal sequencing. This use of a chemical probe led to the rapid discovery of subtilisin-like serine protease of Ophiostoma. Two hypothetical proteins were also captured, with one being a probable endopeptidase K type of protease.

Original languageEnglish
Pages (from-to)937-943
Number of pages7
JournalBiotechnology Letters
Volume29
Issue number6
DOIs
Publication statusPublished - Jun 2007

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