Activity-dependent regulation of synapse and dendritic spine morphology in developing barrel cortex requires phospholipase C-β1 signalling

Tara L. Spires*, Zoltán Molnár, Peter C. Kind, Patricia M. Cordery, A. Louise Upton, Colin Blakemore, Anthony J. Hannan

*Corresponding author for this work

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The phospholipase C-β1 (PLC-β1) signalling pathway, activated via metabotropic glutamate receptors (mGluRs), is implicated in activity-dependent development of the cerebral cortex, as both PLC-β1 and mGluR5 knockout mice exhibit disrupted barrel formation in somatosensory cortex. To characterize the effects of this signalling system on development of synaptic circuitry in barrel cortex, we have examined neuronal ultrastructure, synapse formation and dendritic spine morphology in PLC-β1 knockout mice. Qualitative ultrastructure of neurons and synapse density in layers 2-4 of barrel cortex were unchanged in PLC-β1 knockout mice during development [postnatal day (P) 5] and in mature cortex (P19-21). We found a decrease in the proportion of synapses with symmetric morphology at P5 that was gone by P19-21, indicating a transient imbalance in excitatory and inhibitory circuitry. We also investigated dendritic spines by back-labelling layer 5 pyramidal neurons with carbocyanine. We observed normal dendritic spine densities on apical dendrites as they passed through layer 4 of barrel cortex, but spine morphology was altered in PLC-β1 knockout mice at P9. These observations indicate that the PLC-β1 signalling pathway plays a role in the development of normal cortical circuitry. Interrupting this regulation leads to changes in synapse and dendritic spine morphology, possibly altering post-synaptic integration of signal.

Original languageEnglish
Pages (from-to)385-393
Number of pages9
JournalCerebral Cortex
Volume15
Issue number4
DOIs
Publication statusPublished - Apr 2005
Externally publishedYes

Keywords

  • Cortical development
  • mGluR5
  • PLC-β1
  • Stereology
  • Synapse morphology
  • Synaptogenesis

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    Spires, T. L., Molnár, Z., Kind, P. C., Cordery, P. M., Upton, A. L., Blakemore, C., & Hannan, A. J. (2005). Activity-dependent regulation of synapse and dendritic spine morphology in developing barrel cortex requires phospholipase C-β1 signalling. Cerebral Cortex, 15(4), 385-393. https://doi.org/10.1093/cercor/bhh141