Addition of nitric oxide through nitric oxide-paracetamol enhances healing rat achilles tendon

George A.C. Murrell, Gongyao Tang, Richard C. Appleyard, Piero Del Soldato, Min Xia Wang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Nitric oxide is an important messenger molecule in many physiological processes. The addition of NO via NO-flurbiprofen enhances the material properties of healing tendon, however, flurbiprofen has a detrimental effect on healing. We asked if NO delivered by a cyclooxygenase 3 inhibitor (paracetamol/acetaminophen) would enhance healing in a rat Achilles tendon healing model. Rats were injected subcutaneously daily with NO-paracetamol, paracetamol or vehicle from two days before surgery to the day of tissue harvesting. Paracetamol had no effect on tendon healing compared with vehicle alone. NO-paracetamol did not change the failure load, but did decrease the water content, enhance the collagen content, reduce the cross-sectional area and improve the ultimate stress of healing tendon compared with paracetamol and vehicle. The collagen organization of the healing tendon in the NO-paracetamol group, as determined by polarized light microscopy, was enhanced. Our data suggests NO-paracetamol increases the total collagen content and enhances organization while decreasing the cross-sectional area of healing rat Achilles tendon and is consistent with human clinical trials where NO has improved the symptoms and signs of tendinopathy.

Original languageEnglish
Pages (from-to)1618-1624
Number of pages7
JournalClinical Orthopaedics and Related Research
Volume466
Issue number7
DOIs
Publication statusPublished - Jul 2008
Externally publishedYes

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