Adhesive pad differentiation in Drosophila melanogaster depends on the Polycomb group gene Su(z)2

Mirko Husken, Kim Hufnagel, Katharina Mende, Esther Appel, Heiko Meyer, Henrik Peisker, Markus Togelo, Shuoshuo Wang, Jonas Wolff, Stanislav N. Gorb, Achim Paululat

Research output: Contribution to journalArticle

3 Citations (Scopus)
5 Downloads (Pure)

Abstract

The ability of many insects to walk on vertical smooth surfaces such as glass or even on the ceiling has fascinated biologists for a long time, and has led to the discovery of highly specialized adhesive organs located at the distal end of the animals' legs. So far, research has primarily focused on structural and ultrastructural investigations leading to a deeper understanding of adhesive organ functionality and to the development of new bioinspired materials. Genetic approaches, e.g. the analysis of mutants, to achieve a better understanding of adhesive organ differentiation have not been used so far. Here, we describe the first Drosophila melanogaster mutant that develops malformed adhesive organs, resulting in a complete loss of climbing ability on vertical smooth surfaces. Interestingly, these mutants fail to make close contact between the setal tips and the smooth surface, a crucial condition for wet adhesion mediated by capillary forces. Instead, these flies walk solely on their claws. Moreover, wewere able to show that the mutation is caused by a P-element insertion into the Su(z)2 gene locus. Remobilization of the P-element restores climbing ability. Furthermore, we provide evidence that the P-element insertion results in an artificial Su(z)2 transcript, which most likely causes a gain-of-function mutation. We presume that this transcript causes deregulation of yet unknown target genes involved in pulvilli differentiation. Our results nicely demonstrate that the genetically treatable model organism Drosophila is highly suitable for future investigations on adhesive organ differentiation.

Original languageEnglish
Pages (from-to)1159-1165
Number of pages7
JournalJournal of Experimental Biology
Volume218
Issue number8
DOIs
Publication statusPublished - 15 Apr 2015
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2015. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Correction can be found at Journal of Experimental Biology volume 218(10) p 1613, https://doi.org/10.1242/jeb.125120

Keywords

  • Drosophila
  • Su(z)2
  • adhesive organ
  • pulvillus
  • seta
  • smooth surface

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    Husken, M., Hufnagel, K., Mende, K., Appel, E., Meyer, H., Peisker, H., ... Paululat, A. (2015). Adhesive pad differentiation in Drosophila melanogaster depends on the Polycomb group gene Su(z)2. Journal of Experimental Biology, 218(8), 1159-1165. https://doi.org/10.1242/jeb.108332