TY - JOUR
T1 - Aggregation behavior and size of lipopolysaccharide from Escherichia coli O55:B5
AU - Bergstrand, Anna
AU - Svanberg, Christer
AU - Langton, Maud
AU - Nydén, Magnus
PY - 2006/11/1
Y1 - 2006/11/1
N2 - Dynamic light scattering, steady-state fluorescence, NMR diffusometry and cryo-TEM have been used to gain more insight into the aggregation behaviour of LPS from Escherichia coli O55:B5. Knowledge of this behaviour of the amphiphilic LPS molecule is in many cases of importance for the design of experiments and interpretation of results when using LPS in solution. The aim of this work was to study the aggregation and determine the aggregate size of E. coli O55:B5. The mean hydrodynamic radius of the LPS aggregates was determined by NMR diffusometry and dynamic light scattering to 14 and 26 nm, respectively. The cryo-TEM technique revealed predominately spherical aggregates of 9-19 nm. We wish to report 10 μg/ml as the aggregation start for LPS E. coli O55:B5 in PBS buffer, pH 7.2. We suggest that the aggregation is a continuous process that starts at 10 μg/ml and proceeds up to 300 μg/ml.
AB - Dynamic light scattering, steady-state fluorescence, NMR diffusometry and cryo-TEM have been used to gain more insight into the aggregation behaviour of LPS from Escherichia coli O55:B5. Knowledge of this behaviour of the amphiphilic LPS molecule is in many cases of importance for the design of experiments and interpretation of results when using LPS in solution. The aim of this work was to study the aggregation and determine the aggregate size of E. coli O55:B5. The mean hydrodynamic radius of the LPS aggregates was determined by NMR diffusometry and dynamic light scattering to 14 and 26 nm, respectively. The cryo-TEM technique revealed predominately spherical aggregates of 9-19 nm. We wish to report 10 μg/ml as the aggregation start for LPS E. coli O55:B5 in PBS buffer, pH 7.2. We suggest that the aggregation is a continuous process that starts at 10 μg/ml and proceeds up to 300 μg/ml.
KW - Dynamic light scattering
KW - Lipopolysaccharide
KW - NMR diffusometry
KW - Steady state fluorescence
UR - http://www.scopus.com/inward/record.url?scp=33750065738&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2006.06.007
DO - 10.1016/j.colsurfb.2006.06.007
M3 - Article
C2 - 16934960
AN - SCOPUS:33750065738
SN - 0927-7765
VL - 53
SP - 9
EP - 14
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 1
ER -