Allocating sex in road-killed possums using PCR

Tracey C. Russell; Linda E. Neaves; Catherine A. Herbert

doi:10.1071/AM09028

Allocating sex in road-killed possums using PCR

Tracey C. Russell, Linda E. Neaves, Catherine A. Herbert

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Road-killed animals are a readily available but often overlooked source of specimens and data. Determining the sex of road-killed possums is often difficult due to the severe damage carcasses sustain on impact with vehicles. Consequently, valuable information is lost. To assess the potential application of genetic techniques to address this problem, tissue biopsies (n=47) taken from road-killed possums were sexed via PCR, through the simultaneous use of W- (G6PD) and Y-linked (SRY) primers. Comparisons with animals of known sex were used to validate the accuracy of this method, with 100% (n=19) of samples correctly identified in a blind test. This methodology can provide researchers with valuable, additional data for road-kill studies where assigning sex in the field is not possible.

Original language	English
Pages (from-to)	1-4
Number of pages	4
Journal	Australian Mammalogy
Volume	33
Issue number	1
DOIs	https://doi.org/10.1071/AM09028
Publication status	Published - 2011

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10.1071/AM09028

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title = "Allocating sex in road-killed possums using PCR",

abstract = "Road-killed animals are a readily available but often overlooked source of specimens and data. Determining the sex of road-killed possums is often difficult due to the severe damage carcasses sustain on impact with vehicles. Consequently, valuable information is lost. To assess the potential application of genetic techniques to address this problem, tissue biopsies (n=47) taken from road-killed possums were sexed via PCR, through the simultaneous use of W- (G6PD) and Y-linked (SRY) primers. Comparisons with animals of known sex were used to validate the accuracy of this method, with 100% (n=19) of samples correctly identified in a blind test. This methodology can provide researchers with valuable, additional data for road-kill studies where assigning sex in the field is not possible.",

author = "Russell, {Tracey C.} and Neaves, {Linda E.} and Herbert, {Catherine A.}",

year = "2011",

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AU - Russell, Tracey C.

AU - Neaves, Linda E.

AU - Herbert, Catherine A.

PY - 2011

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N2 - Road-killed animals are a readily available but often overlooked source of specimens and data. Determining the sex of road-killed possums is often difficult due to the severe damage carcasses sustain on impact with vehicles. Consequently, valuable information is lost. To assess the potential application of genetic techniques to address this problem, tissue biopsies (n=47) taken from road-killed possums were sexed via PCR, through the simultaneous use of W- (G6PD) and Y-linked (SRY) primers. Comparisons with animals of known sex were used to validate the accuracy of this method, with 100% (n=19) of samples correctly identified in a blind test. This methodology can provide researchers with valuable, additional data for road-kill studies where assigning sex in the field is not possible.

AB - Road-killed animals are a readily available but often overlooked source of specimens and data. Determining the sex of road-killed possums is often difficult due to the severe damage carcasses sustain on impact with vehicles. Consequently, valuable information is lost. To assess the potential application of genetic techniques to address this problem, tissue biopsies (n=47) taken from road-killed possums were sexed via PCR, through the simultaneous use of W- (G6PD) and Y-linked (SRY) primers. Comparisons with animals of known sex were used to validate the accuracy of this method, with 100% (n=19) of samples correctly identified in a blind test. This methodology can provide researchers with valuable, additional data for road-kill studies where assigning sex in the field is not possible.

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DO - 10.1071/AM09028

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JO - Australian Mammalogy

JF - Australian Mammalogy

IS - 1

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Allocating sex in road-killed possums using PCR

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