Alpha2-macroglobulin from an Atlantic shrimp

Biochemical characterization, sub-cellular localization and gene expression upon fungal challenge

Luciane Maria Perazzolo*, Evelyne Bachère, Rafael Diego Rosa, Priscila Goncalves, Edemar Roberto Andreatta, Sirlei Daffre, Margherita Anna Barracco

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study, we report on the isolation and characterization of an alpha2-macroglobulin (α2M) from the plasma of the pink shrimp Farfantepenaeus paulensis, its sub-cellular localization and transcriptional changes after infection by fungi. The molecular mass of the α2M was estimated at 389 kDa by gel filtration and 197 kDa by SDS-PAGE, under reducing conditions, suggesting that α2M from F. paulensis consists of two identical sub-units, covalently linked by disulphide bonds. The N-terminal amino acid sequence of the α2M from F. paulensis was very similar to those of other penaeid shrimps, crayfish and lobster (70-90% identity) and to a less extent with that of freshwater prawn (40% identity). A monoclonal antibody raised against the Marsupenaeus japonicus α2M made it possible to demonstrate that α2M of F. paulensis is stored in the vesicles of the shrimp granular hemocytes (through immunogold assay). Quantitative real-time PCR (qPCR) analysis showed that α2M mRNA transcripts significantly increased 24 h after an experimental infection with the shrimp pathogen Fusarium solani and it returned to the basal levels at 48 h post-injection. This is the first report on a α2M characterization in an Atlantic penaeid species and its expression profile upon a fungal infection.

Original languageEnglish
Pages (from-to)938-943
Number of pages6
JournalFish and Shellfish Immunology
Volume31
Issue number6
DOIs
Publication statusPublished - Dec 2011

Keywords

  • Alpha2-macroglobulin
  • Farfantepenaeus paulensis
  • Hemocyte
  • Shrimp immunity
  • Transcriptional response to fungal infection

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