Alterations in architecture and metabolism induced by ultraviolet radiation-B in the carragenophyte Chondracanthus teedei (Rhodophyta, Gigartinales)

Éder C. Schmidt, Beatriz Pereira, Carime L Mansur Pontes, Rodrigo dos Santos, Fernando Scherner, Paulo A. Horta, Roberta Paula de Martins, Alexandra Latini, Marcelo Maraschin, Zenilda L. Bouzon

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The in vivo effect of ultraviolet radiation-B (UVBR) in apical segments of Chondracanthus teedei was examined. Over a period of 7 days, the segments were cultivated and exposed to photosynthetically active radiation (PAR) at 80 μmol photons m -2 s -1 and PAR + UVBR at 1.6 W m -2 for 3 h per day. The samples were processed for electron microscopy and histochemistry; also was analyzed growth rates, mitochondrial activity, protein levels, content of photosynthetic pigments and photosynthetic performance. UVBR elicited increased cell wall thickness and accumulation of plastoglobuli, changes in mitochondrial organization and destruction of chloroplast internal organization. Compared to controls, algae exposed to PAR + UVBR showed a growth rate reduction of 55%. The content of photosynthetic pigments, including chlorophyll a and phycobiliproteins, decreased after exposure to PAR + UVBR. This result agrees with the decreased photosynthetic performance observed after exposing algae to PAR + UVBR. Irradiation also elicited increased activity of the antioxidant enzyme glutathione peroxidase and decreased mitochondrial NADH dehydrogenase activity, which correlated with the decreased protein content in plants exposed to PAR + UVBR. Taken together, these findings strongly indicate that UVBR negatively affects the architecture and metabolism of the carragenophyte C. teedei.

Original languageEnglish
Pages (from-to)353-367
Number of pages15
JournalProtoplasma
Volume249
Issue number2
DOIs
Publication statusPublished - Apr 2012
Externally publishedYes

Keywords

  • Chondracanthus teedei
  • Mitochondrial activity
  • Photosynthetic performance
  • Photosynthetic pigments
  • Ultrastructure
  • Ultraviolet radiation-B

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