Alterations to the protein profile of bladder carcinoma cell lines induced by plant extract MINA-05 in vitro

Terry Nguyen-Khuong, Melanie Y. White, Tzong Tyng Hung, Shona Seeto, Melissa L. Thomas, Anna M. Fitzgerald, Carlos E. Martucci, Sharon Luk, Shiu Fu Pang, Pamela J. Russell, Bradley J. Walsh

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Bladder cancer (BLCa) is a severe urological cancer of both men and women that commonly recurs and once invasive, is difficult to treat. MINA-05 (CK Life Sciences Int'l, Hong Kong) is a derivative of complex botanical extracts, shown to reduce cellular proliferation of bladder and prostate carcinomas. We tested the effects of MINA-05 against human BLCa cell sublines, B8, B8-RSP-GCK, B8-RSP-LN and C3, from a transitional cell carcinoma, grade IV, to determine the molecular targets of treatment by observing the cellular protein profile. Cells were acclimatised for 48 h then treated for 72 h with concentrations of MINA-05 reflecting 1/2 IC50, IC50 and 2xIC50 (n = 3) or with vehicle, (0.5% DMSO). Dose-dependant changes in protein abundance were detected and characterised using 2-dimensional electrophoresis and MS. We identified 10 proteins that underwent changes in abundance, pI and/or molecular mass in response to treatment. MINA-05 was shown to influence proteins across numerous functional classes including cytoskeletal proteins, energy metabolism proteins, protein degradation proteins and tumour suppressors, suggesting a global impact on these cell lines. This study implies that the ability of MINA-05 to retard cellular proliferation is attributed to its ability to alter cell cycling, metabolism, protein degradation and the cancer cell environment.

Original languageEnglish
Pages (from-to)1883-1892
Number of pages10
JournalProteomics
Volume9
Issue number7
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

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