Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum

Alexandr Kurkov; Anna Guller; Alexey Fayzullin; Nafisa Fayzullina; Vladimir Plyakin; Svetlana Kotova; Petr Timashev; Anastasia Frolova; Nikita Kurtak; Vyacheslav Paukov; Anatoly Shekhter

doi:10.1371/journal.pone.0245159

Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum

Alexandr Kurkov, Anna Guller, Alexey Fayzullin^*, Nafisa Fayzullina, Vladimir Plyakin, Svetlana Kotova, Petr Timashev, Anastasia Frolova, Nikita Kurtak, Vyacheslav Paukov, Anatoly Shekhter

^*Corresponding author for this work

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Abstract

Background It is unclear if amianthoid transformation (AT) of costal cartilage extracellular matrix (ECM) has an impact on the development of pectus excavatum (PE) and pectus carinatum (PC). Methods AT foci were examined in intrasurgical biopsy specimens of costal cartilages of children (8–17 years old) with PE (n = 12) and PC (n = 12) and in age-matching autopsy control samples (n = 10) using histological and immunohistochemical staining, atomic force and nonlinear optical microscopy, transmission and scanning electron microscopy, morphometry and statistics. Results AT areas were identified in the costal cartilage ECM in children with normal chest, PE and PC. Each type of the AT areas (“canonical”, “intertwined”, “fine-fibred” and “intralacunary”) had a unique morphological pattern of thickness and alignment of amianthoid fibers (AFs). AFs were formed via lateral aggregation of collagen type II fibrils in the intact ECM. Foci of the AT were observed significantly more frequently in the PE and PC groups. The AT areas had unique quantitative features in each study group. Conclusion AT is a structurally diverse form of ECM alteration present in healthy and pathological costal cartilage. PE and PC are associated with specific AT disorders.

Original language	English
Article number	e0245159
Pages (from-to)	1-25
Number of pages	25
Journal	PLoS ONE
Volume	16
Issue number	1 January
DOIs	https://doi.org/10.1371/journal.pone.0245159
Publication status	Published - 25 Jan 2021
Externally published	Yes

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Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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Kurkov, Alexandr ; Guller, Anna ; Fayzullin, Alexey ; Fayzullina, Nafisa ; Plyakin, Vladimir ; Kotova, Svetlana ; Timashev, Petr ; Frolova, Anastasia ; Kurtak, Nikita ; Paukov, Vyacheslav ; Shekhter, Anatoly. / Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum. In: PLoS ONE. 2021 ; Vol. 16, No. 1 January. pp. 1-25.

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title = "Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum",

abstract = "Background It is unclear if amianthoid transformation (AT) of costal cartilage extracellular matrix (ECM) has an impact on the development of pectus excavatum (PE) and pectus carinatum (PC). Methods AT foci were examined in intrasurgical biopsy specimens of costal cartilages of children (8–17 years old) with PE (n = 12) and PC (n = 12) and in age-matching autopsy control samples (n = 10) using histological and immunohistochemical staining, atomic force and nonlinear optical microscopy, transmission and scanning electron microscopy, morphometry and statistics. Results AT areas were identified in the costal cartilage ECM in children with normal chest, PE and PC. Each type of the AT areas (“canonical”, “intertwined”, “fine-fibred” and “intralacunary”) had a unique morphological pattern of thickness and alignment of amianthoid fibers (AFs). AFs were formed via lateral aggregation of collagen type II fibrils in the intact ECM. Foci of the AT were observed significantly more frequently in the PE and PC groups. The AT areas had unique quantitative features in each study group. Conclusion AT is a structurally diverse form of ECM alteration present in healthy and pathological costal cartilage. PE and PC are associated with specific AT disorders.",

author = "Alexandr Kurkov and Anna Guller and Alexey Fayzullin and Nafisa Fayzullina and Vladimir Plyakin and Svetlana Kotova and Petr Timashev and Anastasia Frolova and Nikita Kurtak and Vyacheslav Paukov and Anatoly Shekhter",

note = "Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",

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Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum. / Kurkov, Alexandr; Guller, Anna; Fayzullin, Alexey; Fayzullina, Nafisa; Plyakin, Vladimir; Kotova, Svetlana; Timashev, Petr; Frolova, Anastasia; Kurtak, Nikita; Paukov, Vyacheslav; Shekhter, Anatoly.

In: PLoS ONE, Vol. 16, No. 1 January, e0245159, 25.01.2021, p. 1-25.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum

AU - Kurkov, Alexandr

AU - Guller, Anna

AU - Fayzullin, Alexey

AU - Fayzullina, Nafisa

AU - Plyakin, Vladimir

AU - Kotova, Svetlana

AU - Timashev, Petr

AU - Frolova, Anastasia

AU - Kurtak, Nikita

AU - Paukov, Vyacheslav

AU - Shekhter, Anatoly

N1 - Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2021/1/25

Y1 - 2021/1/25

N2 - Background It is unclear if amianthoid transformation (AT) of costal cartilage extracellular matrix (ECM) has an impact on the development of pectus excavatum (PE) and pectus carinatum (PC). Methods AT foci were examined in intrasurgical biopsy specimens of costal cartilages of children (8–17 years old) with PE (n = 12) and PC (n = 12) and in age-matching autopsy control samples (n = 10) using histological and immunohistochemical staining, atomic force and nonlinear optical microscopy, transmission and scanning electron microscopy, morphometry and statistics. Results AT areas were identified in the costal cartilage ECM in children with normal chest, PE and PC. Each type of the AT areas (“canonical”, “intertwined”, “fine-fibred” and “intralacunary”) had a unique morphological pattern of thickness and alignment of amianthoid fibers (AFs). AFs were formed via lateral aggregation of collagen type II fibrils in the intact ECM. Foci of the AT were observed significantly more frequently in the PE and PC groups. The AT areas had unique quantitative features in each study group. Conclusion AT is a structurally diverse form of ECM alteration present in healthy and pathological costal cartilage. PE and PC are associated with specific AT disorders.

AB - Background It is unclear if amianthoid transformation (AT) of costal cartilage extracellular matrix (ECM) has an impact on the development of pectus excavatum (PE) and pectus carinatum (PC). Methods AT foci were examined in intrasurgical biopsy specimens of costal cartilages of children (8–17 years old) with PE (n = 12) and PC (n = 12) and in age-matching autopsy control samples (n = 10) using histological and immunohistochemical staining, atomic force and nonlinear optical microscopy, transmission and scanning electron microscopy, morphometry and statistics. Results AT areas were identified in the costal cartilage ECM in children with normal chest, PE and PC. Each type of the AT areas (“canonical”, “intertwined”, “fine-fibred” and “intralacunary”) had a unique morphological pattern of thickness and alignment of amianthoid fibers (AFs). AFs were formed via lateral aggregation of collagen type II fibrils in the intact ECM. Foci of the AT were observed significantly more frequently in the PE and PC groups. The AT areas had unique quantitative features in each study group. Conclusion AT is a structurally diverse form of ECM alteration present in healthy and pathological costal cartilage. PE and PC are associated with specific AT disorders.

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VL - 16

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JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 1 January

M1 - e0245159

ER -

Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum

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