TY - JOUR
T1 - Amide proton exchange in human metallothionein-2 measured by nuclear magnetic resonance spectroscopy
AU - Messerle, Barbara A.
AU - Bos, Martin
AU - Schäffer, Andreas
AU - Vašák, Milan
AU - Kägi, Jeremias H R
AU - Wüthrich, Kurt
PY - 1990/8/5
Y1 - 1990/8/5
N2 - In human metallothionein-2, the exchange rate constants of ten amide protons were found to range from 1.7 × 10-4 to 1 × 10-1 min-1 at pH 6.3 and 8 °C. Most of these slowly exchanging protons could be associated with hydrogen bonds in secondary structure elements of the α-domain. Amide proton exchange rates thus present an additional criterion for the structural characterization of different metallothioneins, which could be particularly valuable for comparisons of different homologous protein preparations containing nuclear magnetic resonance-inactive metal ions, where the metal-polypeptide co-ordinative bonds cannot be identified directly.
AB - In human metallothionein-2, the exchange rate constants of ten amide protons were found to range from 1.7 × 10-4 to 1 × 10-1 min-1 at pH 6.3 and 8 °C. Most of these slowly exchanging protons could be associated with hydrogen bonds in secondary structure elements of the α-domain. Amide proton exchange rates thus present an additional criterion for the structural characterization of different metallothioneins, which could be particularly valuable for comparisons of different homologous protein preparations containing nuclear magnetic resonance-inactive metal ions, where the metal-polypeptide co-ordinative bonds cannot be identified directly.
UR - http://www.scopus.com/inward/record.url?scp=0025163762&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(90)90292-T
DO - 10.1016/0022-2836(90)90292-T
M3 - Article
C2 - 2167380
AN - SCOPUS:0025163762
SN - 0022-2836
VL - 214
SP - 781
EP - 786
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 3
ER -