Amino acid analysis using derivatisation with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography

Paul A. Haynes*, David Sheumack, Jeffrey Kibby, John W. Redmond

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    90 Citations (Scopus)

    Abstract

    A simple procedure for the precolumn derivatisation of amino acids with 9-fluorenylmethyl chloroformate and a liquid chromatographic method for the separation of the derivatives with fluorimetric detection in the picomole range are reported. The procedure does not involve a solvent extraction and gives single, stable derivatives of the common protein amino acids. The method has been demonstrated on hydrolysates of proteins and peptides.

    Original languageEnglish
    Pages (from-to)177-185
    Number of pages9
    JournalJournal of Chromatography A
    Volume540
    Issue numberC
    DOIs
    Publication statusPublished - 1991

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