Abstract
A simple procedure for the precolumn derivatisation of amino acids with 9-fluorenylmethyl chloroformate and a liquid chromatographic method for the separation of the derivatives with fluorimetric detection in the picomole range are reported. The procedure does not involve a solvent extraction and gives single, stable derivatives of the common protein amino acids. The method has been demonstrated on hydrolysates of proteins and peptides.
Original language | English |
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Pages (from-to) | 177-185 |
Number of pages | 9 |
Journal | Journal of Chromatography A |
Volume | 540 |
Issue number | C |
DOIs | |
Publication status | Published - 1991 |