Amplified detection signal at a photoelectrochemical aptasensor with a poly(diphenylbutadiene)-BiOBr heterojunction and Au-modified CeO₂ octahedrons

A major aspect of this work is the synergistic application of a poly(diphenylbutadiene)-BiOBr composite and a gold nanoparticle-linked CeO₂ octahedron to develop a photoelectrochemical aptasensor with an easily measurable detection signal change. Specifically, poly(diphenylbutadiene) nanofiber-immobilised BiOBr flower-like microspheres were developed as a hybrid material with a heterojunction that facilitates high visible light absorption and efficient photo-generated charge separation, which are essential features for sensitive photoelectrochemical sensors. The model analyte acetamiprid was attached via its specific aptamer on the aptasensor. Separately, a gold nanoparticle-linked CeO₂ octahedron was strategically used to significantly diminish the photocurrent by impeding electron transfer at the aptasensor surface. After acetamiprid binding, the CeO₂ octahedrons were displaced from the aptasensor. This caused a weakened quenching effect and restored the photocurrent to accomplish an “on-off-on” detection mechanism. This photoelectrochemical aptasensor exhibited a detection limit of 0.05 pM over a linear range of 0.1 pM–10 μM acetamiprid. The use of an aptamer has provided good specificity to acetamiprid and anti-interference. In addition, an ∼5.8% relative standard deviation was estimated as the reproducibility of the photoelectrochemical aptasensor. Furthermore, nearly 90% of the initial photocurrent was still measurable after storing these aptasensors at room temperature for 4 weeks, demonstrating their stability.