Amplified electrochemical aptasensor taking AuNPs based sandwich sensing platform as a model

Bingling Li, Yuling Wang, Hui Wei, Shaojun Dong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

116 Citations (Scopus)


Here, we report a sensitive amplified electrochemical impedimetric aptasensor for thrombin, a kind of serine protease that plays important role in thrombosis and haemostasis. For improving detection sensitivity, a sandwich sensing platform is fabricated, in which the thiolated aptamers are firstly immobilized on a gold substrate to capture the thrombin molecules, and then the aptamer functionalized Au nanoparticles (AuNPs) are used to amplify the impedimetric signals. Such designed aptamer/thrombin/AuNPs sensing system could not only improve the detection sensitivity compared to the reported impedimetric aptasensors but also provide a promising signal amplified model for aptamer-based protein detection. In this paper, we realize a sensitive detection limit of 0.02 nM, with a linear range of 0.05-18 nM. Meanwhile, the effect of 6-mercaptohexanol (MCH) and 2-mercaptoethanol (MCE) on the modification of the electrode is investigated.

Original languageEnglish
Pages (from-to)965-970
Number of pages6
JournalBiosensors and Bioelectronics
Issue number7
Publication statusPublished - 28 Feb 2008
Externally publishedYes


  • Aptamer
  • Au nanoparticles
  • Impedimetric aptasensor
  • Thrombin


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