Amplified oxygen reduction signal at a Pt-Sn-modified TiO2 nancomposite on an electrochemical aptasensor

Lele Li, Xiaoqiang Liu, Liwei Yang, Si Zhang, HeJie Zheng, Yunfei Tang, Danny K. Y. Wong

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In this work, a metallic composite with strong electrocatalytic property was designed by uniformly decorating Pt and Sn nanoparticles on the surface of TiO2 nanorods (Pt–Sn@TiO2). A detection scheme was then developed based on a dual signal amplification strategy involving the Pt–Sn@TiO2 composite and exonuclease assisted target recycling. The Pt–Sn@TiO2 composite exhibited an enhanced oxygen reduction current owing to the synergistic effect between Pt and Sn, as well as high exposure of Pt (111) crystal face. Initially, a Pt–Sn@TiO2 modified glassy carbon electrode produced an amplified electrochemical signal for the reduction of dissolved oxygen in the analyte solution. Next, a DNA with a complementary sequence to a streptomycin aptamer (cDNA) was immobilised on the Pt–Sn@TiO2 modified electrode, followed by the streptomycin aptamer that hybridised with cDNA. The corresponding oxygen reduction current was diminished by 51% attributable to the hindrance from the biomolecules. After a mixture of streptomycin and RecJf exonuclease was introduced, both the streptomycin-aptamer complex and the cDNA were cleaved from the electrode, making the Pt–Sn and Pt (111) surface available for oxygen reduction. RecJf would also release streptomycin from the streptomycin-aptamer complex, allowing it to complex again with aptamers on the electrode. This has then promoted a cyclic amplification of the oxygen reduction current by 85%, which is quantitatively related to streptomycin. Under optimal conditions, the aptasensor exhibited a linear range of 0.05–1500 nM and a limit of detection of 0.02±0.0045 nM streptomycin. The sensor was then used in the real-life sample detection of streptomycin to demonstrate its potential applications to bioanalysis.
LanguageEnglish
Article number111525
Number of pages10
JournalBiosensors and Bioelectronics
Volume142
DOIs
Publication statusPublished - 1 Oct 2019

Fingerprint

Streptomycin
Oxygen
Electrodes
Amplification
Composite materials
Exonucleases
Complementary DNA
Glassy carbon
Biomolecules
Dissolved oxygen
Nanorods
Recycling
DNA
Nanotubes
Nanoparticles
Crystals
Sensors
Limit of Detection
Carbon

Keywords

  • Pt-Sn@TiO₂ composite
  • Oxygen reduction reaction
  • Electrochemical aptasensor
  • RecJf exonuclease
  • Streptomycin

Cite this

Li, Lele ; Liu, Xiaoqiang ; Yang, Liwei ; Zhang, Si ; Zheng, HeJie ; Tang, Yunfei ; Wong, Danny K. Y. / Amplified oxygen reduction signal at a Pt-Sn-modified TiO2 nancomposite on an electrochemical aptasensor. In: Biosensors and Bioelectronics. 2019 ; Vol. 142.
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title = "Amplified oxygen reduction signal at a Pt-Sn-modified TiO2 nancomposite on an electrochemical aptasensor",
abstract = "In this work, a metallic composite with strong electrocatalytic property was designed by uniformly decorating Pt and Sn nanoparticles on the surface of TiO2 nanorods (Pt–Sn@TiO2). A detection scheme was then developed based on a dual signal amplification strategy involving the Pt–Sn@TiO2 composite and exonuclease assisted target recycling. The Pt–Sn@TiO2 composite exhibited an enhanced oxygen reduction current owing to the synergistic effect between Pt and Sn, as well as high exposure of Pt (111) crystal face. Initially, a Pt–Sn@TiO2 modified glassy carbon electrode produced an amplified electrochemical signal for the reduction of dissolved oxygen in the analyte solution. Next, a DNA with a complementary sequence to a streptomycin aptamer (cDNA) was immobilised on the Pt–Sn@TiO2 modified electrode, followed by the streptomycin aptamer that hybridised with cDNA. The corresponding oxygen reduction current was diminished by 51{\%} attributable to the hindrance from the biomolecules. After a mixture of streptomycin and RecJf exonuclease was introduced, both the streptomycin-aptamer complex and the cDNA were cleaved from the electrode, making the Pt–Sn and Pt (111) surface available for oxygen reduction. RecJf would also release streptomycin from the streptomycin-aptamer complex, allowing it to complex again with aptamers on the electrode. This has then promoted a cyclic amplification of the oxygen reduction current by 85{\%}, which is quantitatively related to streptomycin. Under optimal conditions, the aptasensor exhibited a linear range of 0.05–1500 nM and a limit of detection of 0.02±0.0045 nM streptomycin. The sensor was then used in the real-life sample detection of streptomycin to demonstrate its potential applications to bioanalysis.",
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Amplified oxygen reduction signal at a Pt-Sn-modified TiO2 nancomposite on an electrochemical aptasensor. / Li, Lele; Liu, Xiaoqiang; Yang, Liwei; Zhang, Si; Zheng, HeJie; Tang, Yunfei; Wong, Danny K. Y.

In: Biosensors and Bioelectronics, Vol. 142, 111525, 01.10.2019.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Amplified oxygen reduction signal at a Pt-Sn-modified TiO2 nancomposite on an electrochemical aptasensor

AU - Li, Lele

AU - Liu, Xiaoqiang

AU - Yang, Liwei

AU - Zhang, Si

AU - Zheng, HeJie

AU - Tang, Yunfei

AU - Wong, Danny K. Y.

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KW - Oxygen reduction reaction

KW - Electrochemical aptasensor

KW - RecJf exonuclease

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