Amyloid β induces early changes in the ribosomal machinery, cytoskeletal organization and oxidative phosphorylation in retinal photoreceptor cells

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Abstract

Amyloid β (Aβ) accumulation and its aggregation is characteristic molecular feature of the development of Alzheimer's disease (AD). More recently, Aβ has been suggested to be associated with retinal pathology associated with AD, glaucoma and drusen deposits in age related macular degeneration (AMD). In this study, we investigated the proteins and biochemical networks that are affected by Aβ in the 661 W photoreceptor cells in culture. Time and dose dependent effects of Aβ on the photoreceptor cells were determined utilizing tandem mass tag (TMT) labeling-based quantitative mass-spectrometric approach. Bioinformatic analysis of the data revealed concentration and time dependent effects of the Aβ peptide stimulation on various key biochemical pathways that might be involved in mediating the toxicity effects of the peptide. We identified increased Tau phosphorylation, GSK3β dysregulation and reduced cell viability in cells treated with Aβ in a dose and time dependent manner. This study has delineated for the first-time molecular networks in photoreceptor cells that are impacted early upon Aβ treatment and contrasted the findings with a longer-term treatment effect. Proteins associated with ribosomal machinery homeostasis, mitochondrial function and cytoskeletal organization were affected in the initial stages of Aβ exposure, which may provide key insights into AD effects on the photoreceptors and specific molecular changes induced by Aβ peptide.

LanguageEnglish
Article number24
Number of pages14
JournalFrontiers in Molecular Neuroscience
Volume12
DOIs
Publication statusPublished - 22 Feb 2019

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Vertebrate Photoreceptor Cells
Oxidative Phosphorylation
Amyloid
Photoreceptor Cells
Alzheimer Disease
Amyloid beta-Peptides
Macular Degeneration
Computational Biology
Glaucoma
Cell Survival
Proteins
Homeostasis
Cell Culture Techniques
Phosphorylation
Pathology
Peptides

Bibliographical note

Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • proteomics
  • TMT
  • Alzheimer's disease
  • photoreceptor
  • autophagy
  • amyloid
  • retina

Cite this

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title = "Amyloid β induces early changes in the ribosomal machinery, cytoskeletal organization and oxidative phosphorylation in retinal photoreceptor cells",
abstract = "Amyloid β (Aβ) accumulation and its aggregation is characteristic molecular feature of the development of Alzheimer's disease (AD). More recently, Aβ has been suggested to be associated with retinal pathology associated with AD, glaucoma and drusen deposits in age related macular degeneration (AMD). In this study, we investigated the proteins and biochemical networks that are affected by Aβ in the 661 W photoreceptor cells in culture. Time and dose dependent effects of Aβ on the photoreceptor cells were determined utilizing tandem mass tag (TMT) labeling-based quantitative mass-spectrometric approach. Bioinformatic analysis of the data revealed concentration and time dependent effects of the Aβ peptide stimulation on various key biochemical pathways that might be involved in mediating the toxicity effects of the peptide. We identified increased Tau phosphorylation, GSK3β dysregulation and reduced cell viability in cells treated with Aβ in a dose and time dependent manner. This study has delineated for the first-time molecular networks in photoreceptor cells that are impacted early upon Aβ treatment and contrasted the findings with a longer-term treatment effect. Proteins associated with ribosomal machinery homeostasis, mitochondrial function and cytoskeletal organization were affected in the initial stages of Aβ exposure, which may provide key insights into AD effects on the photoreceptors and specific molecular changes induced by Aβ peptide.",
keywords = "proteomics, TMT, Alzheimer's disease, photoreceptor, autophagy, amyloid, retina",
author = "Liting Deng and Kanishka Pushpitha and Chitra Joseph and Veer Gupta and Rashi Rajput and Nitin Chitranshi and Yogita Dheer and Ardeshir Amirkhani and Karthik Kamath and Dana Pascovici and Wu, {Jemma X.} and Salekdeh, {Ghasem Hosseini} and Haynes, {Paul A.} and Graham, {Stuart L.} and Gupta, {Vivek K.} and Mehdi Mirzaei",
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AU - Deng,Liting

AU - Pushpitha,Kanishka

AU - Joseph,Chitra

AU - Gupta,Veer

AU - Rajput,Rashi

AU - Chitranshi,Nitin

AU - Dheer,Yogita

AU - Amirkhani,Ardeshir

AU - Kamath,Karthik

AU - Pascovici,Dana

AU - Wu,Jemma X.

AU - Salekdeh,Ghasem Hosseini

AU - Haynes,Paul A.

AU - Graham,Stuart L.

AU - Gupta,Vivek K.

AU - Mirzaei,Mehdi

N1 - Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2019/2/22

Y1 - 2019/2/22

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