An amperometric immunosensor based on a gold nanoparticle-diazonium salt modified sensing interface for the detection of hba1c in human blood

Guozhen Liu, Sridhar G. Iyengar, J. Justin Gooding*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

An amperometric immunosensor for glycosylated hemoglobin (HbA1c) is reported. A glassy carbon electrode is modified with gold nanoparticles (AuNPs) bearing a ferrocene derivative and a glycosylated pentapeptide (GPP) as an epitope to which anti-HbA1c IgG can selectively bind. The rest of the electrode is passivated with an oligo(ethylene oxide) species to give the electrode resistant to nonspecific adsorption of proteins. Complexation of anti-HbA1c IgG with the surface bound epitope resulted in attenuation of the ferrocene electrochemistry. The immunosensor can detect HbA1c in the range of 4.6%-15.1% of total hemoglobin in human blood by a competitive inhibition assay.

Original languageEnglish
Pages (from-to)881-887
Number of pages7
JournalElectroanalysis
Volume25
Issue number4
DOIs
Publication statusPublished - Apr 2013
Externally publishedYes

Keywords

  • amperometric immunosensor
  • aryl diazonium salts
  • functionalized gold nanoparticles
  • HbA1c

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