An amperometric immunosensor based on a gold nanoparticle-diazonium salt modified sensing interface for the detection of hba1c in human blood

Guozhen Liu; Sridhar G. Iyengar; J. Justin Gooding

doi:10.1002/elan.201200333

An amperometric immunosensor based on a gold nanoparticle-diazonium salt modified sensing interface for the detection of hba1c in human blood

Guozhen Liu, Sridhar G. Iyengar, J. Justin Gooding^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

An amperometric immunosensor for glycosylated hemoglobin (HbA1c) is reported. A glassy carbon electrode is modified with gold nanoparticles (AuNPs) bearing a ferrocene derivative and a glycosylated pentapeptide (GPP) as an epitope to which anti-HbA1c IgG can selectively bind. The rest of the electrode is passivated with an oligo(ethylene oxide) species to give the electrode resistant to nonspecific adsorption of proteins. Complexation of anti-HbA1c IgG with the surface bound epitope resulted in attenuation of the ferrocene electrochemistry. The immunosensor can detect HbA1c in the range of 4.6%-15.1% of total hemoglobin in human blood by a competitive inhibition assay.

Original language	English
Pages (from-to)	881-887
Number of pages	7
Journal	Electroanalysis
Volume	25
Issue number	4
DOIs	https://doi.org/10.1002/elan.201200333
Publication status	Published - Apr 2013
Externally published	Yes

Keywords

amperometric immunosensor
aryl diazonium salts
functionalized gold nanoparticles
HbA1c

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10.1002/elan.201200333

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title = "An amperometric immunosensor based on a gold nanoparticle-diazonium salt modified sensing interface for the detection of hba1c in human blood",

abstract = "An amperometric immunosensor for glycosylated hemoglobin (HbA1c) is reported. A glassy carbon electrode is modified with gold nanoparticles (AuNPs) bearing a ferrocene derivative and a glycosylated pentapeptide (GPP) as an epitope to which anti-HbA1c IgG can selectively bind. The rest of the electrode is passivated with an oligo(ethylene oxide) species to give the electrode resistant to nonspecific adsorption of proteins. Complexation of anti-HbA1c IgG with the surface bound epitope resulted in attenuation of the ferrocene electrochemistry. The immunosensor can detect HbA1c in the range of 4.6%-15.1% of total hemoglobin in human blood by a competitive inhibition assay.",

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AB - An amperometric immunosensor for glycosylated hemoglobin (HbA1c) is reported. A glassy carbon electrode is modified with gold nanoparticles (AuNPs) bearing a ferrocene derivative and a glycosylated pentapeptide (GPP) as an epitope to which anti-HbA1c IgG can selectively bind. The rest of the electrode is passivated with an oligo(ethylene oxide) species to give the electrode resistant to nonspecific adsorption of proteins. Complexation of anti-HbA1c IgG with the surface bound epitope resulted in attenuation of the ferrocene electrochemistry. The immunosensor can detect HbA1c in the range of 4.6%-15.1% of total hemoglobin in human blood by a competitive inhibition assay.

KW - amperometric immunosensor

KW - aryl diazonium salts

KW - functionalized gold nanoparticles

KW - HbA1c

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An amperometric immunosensor based on a gold nanoparticle-diazonium salt modified sensing interface for the detection of hba1c in human blood

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