An assessment of actinobacterial diversity in the marine environment

Paul R. Jensen*, Federico M. Lauro

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

The 16S rRNA gene sequence diversity within the Phylum Actinobacteria was assessed from four sources: PCR-generated V6 sequence tags derived from seawater samples, metagenomic data from the Global Ocean Sampling (GOS) expedition, marine-derived sequences maintained in the Ribosomal Database Project (RDP), and select cultured strains for which sequence data is not yet available in the RDP. This meta-analysis revealed remarkable levels of phylogenetic diversity and confirms the existence of major, deeply rooted, and as of yet uncharacterized lineages within the phylum. A dramatic incongruence among cultured strains and those detected using culture-independent techniques was also revealed. Redundancy among the actinobacteria detected using culture-independent techniques suggests that greater sequence coverage or improved DNA extraction efficiencies may be required to detect the rare phylotypes that can be readily cultured from marine samples. Conversely, new strategies need to be developed for the cultivation of frequently observed but yet to be cultured marine actinobacteria.

Original languageEnglish
Pages (from-to)51-62
Number of pages12
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume94
Issue number1
DOIs
Publication statusPublished - Jun 2008
Externally publishedYes

Keywords

  • Actinomycetes
  • Bacterial diversity
  • Marine actinobacteria
  • Metagenomics

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