TY - JOUR
T1 - An efficient nano-biocatalyst for lignocellulosic biomass hydrolysis
T2 - xylanase immobilization on organically modified biogenic mesoporous silica nanoparticles
AU - Ariaeenejad, Shohreh
AU - Jokar, Farzaneh
AU - Hadian, Parvin
AU - Ma'mani, Leila
AU - Gharaghani, Sajjad
AU - Fereidoonnezhad, Masood
AU - Salekdeh, Ghasem Hosseini
PY - 2020/12/1
Y1 - 2020/12/1
N2 - A biogenic mesoporous silica nanoparticles (MSNs)-based nanocarrier has been used for improving the stability and recyclability of PersiXyn2 as a recombinant xylanase enzyme. The biogenic MSNs (called RKIT-6 henceforth) were synthesized via a soft templating method using rice husk biomass as a renewable silica source. Then bis-(2-aminoethyl) ether modified RKIT-6 (denoted as bis-AE@RKIT-6) was prepared through the furnishing surface with bis-(2-aminoethyl) ether, as a pendant anchoring agent to immobilize PersiXyn2. The nanomaterials were characterized using nitrogen adsorption-desorption isotherms, atomic force microscopy (AFM), X-ray diffraction (XRD), molecular docking (MD) study, and thermogravimetric analysis (TGA). After immobilizing, PersiXyn2@bis-AE@RKIT-6, the optimal temperature of enzyme performance was improved more than 10 °C in comparison with the free enzyme. Such a way that PersiXyn2@bis-AE@RKIT-6 sample could maintain 90% of its maximum activity at the range of 30–60 °C. PersiXyn2@bis-AE@RKIT-6 also enhanced the degradation of lignocellulosic agro-waste (rice straw) and reducing sugar production up to 35% in comparison to the free enzyme. Moreover, PersiXyn2@bis-AE@RKIT-6 could be recycled for ninth runs with a reasonable decrease in its activity. This study presents an efficient nano-biocatalyst which in a more comprehensive sense can be considered as a promising candidate in the fields of animal feed and lignocellulosic biomasses saccharification.
AB - A biogenic mesoporous silica nanoparticles (MSNs)-based nanocarrier has been used for improving the stability and recyclability of PersiXyn2 as a recombinant xylanase enzyme. The biogenic MSNs (called RKIT-6 henceforth) were synthesized via a soft templating method using rice husk biomass as a renewable silica source. Then bis-(2-aminoethyl) ether modified RKIT-6 (denoted as bis-AE@RKIT-6) was prepared through the furnishing surface with bis-(2-aminoethyl) ether, as a pendant anchoring agent to immobilize PersiXyn2. The nanomaterials were characterized using nitrogen adsorption-desorption isotherms, atomic force microscopy (AFM), X-ray diffraction (XRD), molecular docking (MD) study, and thermogravimetric analysis (TGA). After immobilizing, PersiXyn2@bis-AE@RKIT-6, the optimal temperature of enzyme performance was improved more than 10 °C in comparison with the free enzyme. Such a way that PersiXyn2@bis-AE@RKIT-6 sample could maintain 90% of its maximum activity at the range of 30–60 °C. PersiXyn2@bis-AE@RKIT-6 also enhanced the degradation of lignocellulosic agro-waste (rice straw) and reducing sugar production up to 35% in comparison to the free enzyme. Moreover, PersiXyn2@bis-AE@RKIT-6 could be recycled for ninth runs with a reasonable decrease in its activity. This study presents an efficient nano-biocatalyst which in a more comprehensive sense can be considered as a promising candidate in the fields of animal feed and lignocellulosic biomasses saccharification.
KW - Biogenic mesoporous silica nanoparticles
KW - Immobilized enzyme
KW - Xylanase
UR - http://www.scopus.com/inward/record.url?scp=85090338898&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2020.08.211
DO - 10.1016/j.ijbiomac.2020.08.211
M3 - Article
C2 - 32888986
AN - SCOPUS:85090338898
VL - 164
SP - 3462
EP - 3473
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
SN - 0141-8130
ER -