An improved microindentation technique to measure changes in properties of arterial intima during atherogenesis

Barry S. Gow; Wayne D. Castle; Michael J. Legg

doi:10.1016/0021-9290(83)90077-5

An improved microindentation technique to measure changes in properties of arterial intima during atherogenesis

Barry S. Gow^*, Wayne D. Castle, Michael J. Legg

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

A fully automated instrument was developed that measured the amount and time-course of indentation of arterial intima by a spherical tipped probe 25 μm in diameter loaded routinely by forces of 10 μN. The relationship of depth of indentation to time was non-exponential but reached an apparent asymptotic value in about 6 s. Routine indentations were recorded 10 s after loading, the relationship of indentation and force being essentially linear between 0-100 μN. The instrument is an order of magnitude more sensitive than one previously described (Gow and Vaishnav, 1975, J. appl. Physiol. 38, 344-350) and produces indentations of 1-4 μm in the intima of rabbit thoracic aorta with a reproducibility of 1-2%. Preliminary usage of the new microindentor has shown increases in the compliance of the thoracic aorta intima in rabbits during cholesterol feeding.

Original language	English
Pages (from-to)	451-458
Number of pages	8
Journal	Journal of Biomechanics
Volume	16
Issue number	6
DOIs	https://doi.org/10.1016/0021-9290(83)90077-5
Publication status	Published - 1983
Externally published	Yes

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10.1016/0021-9290(83)90077-5

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title = "An improved microindentation technique to measure changes in properties of arterial intima during atherogenesis",

abstract = "A fully automated instrument was developed that measured the amount and time-course of indentation of arterial intima by a spherical tipped probe 25 μm in diameter loaded routinely by forces of 10 μN. The relationship of depth of indentation to time was non-exponential but reached an apparent asymptotic value in about 6 s. Routine indentations were recorded 10 s after loading, the relationship of indentation and force being essentially linear between 0-100 μN. The instrument is an order of magnitude more sensitive than one previously described (Gow and Vaishnav, 1975, J. appl. Physiol. 38, 344-350) and produces indentations of 1-4 μm in the intima of rabbit thoracic aorta with a reproducibility of 1-2%. Preliminary usage of the new microindentor has shown increases in the compliance of the thoracic aorta intima in rabbits during cholesterol feeding.",

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T1 - An improved microindentation technique to measure changes in properties of arterial intima during atherogenesis

AU - Gow, Barry S.

AU - Castle, Wayne D.

AU - Legg, Michael J.

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N2 - A fully automated instrument was developed that measured the amount and time-course of indentation of arterial intima by a spherical tipped probe 25 μm in diameter loaded routinely by forces of 10 μN. The relationship of depth of indentation to time was non-exponential but reached an apparent asymptotic value in about 6 s. Routine indentations were recorded 10 s after loading, the relationship of indentation and force being essentially linear between 0-100 μN. The instrument is an order of magnitude more sensitive than one previously described (Gow and Vaishnav, 1975, J. appl. Physiol. 38, 344-350) and produces indentations of 1-4 μm in the intima of rabbit thoracic aorta with a reproducibility of 1-2%. Preliminary usage of the new microindentor has shown increases in the compliance of the thoracic aorta intima in rabbits during cholesterol feeding.

AB - A fully automated instrument was developed that measured the amount and time-course of indentation of arterial intima by a spherical tipped probe 25 μm in diameter loaded routinely by forces of 10 μN. The relationship of depth of indentation to time was non-exponential but reached an apparent asymptotic value in about 6 s. Routine indentations were recorded 10 s after loading, the relationship of indentation and force being essentially linear between 0-100 μN. The instrument is an order of magnitude more sensitive than one previously described (Gow and Vaishnav, 1975, J. appl. Physiol. 38, 344-350) and produces indentations of 1-4 μm in the intima of rabbit thoracic aorta with a reproducibility of 1-2%. Preliminary usage of the new microindentor has shown increases in the compliance of the thoracic aorta intima in rabbits during cholesterol feeding.

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An improved microindentation technique to measure changes in properties of arterial intima during atherogenesis

Abstract

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