TY - JOUR
T1 - An in vitro evidence for caffeic acid, rosmarinic acid and trans cinnamic acid as a skin protectant against γ-radiation
AU - Hakkim, Faruck Lukmanul
AU - Miura, Miwa
AU - Matsuda, Naoki
AU - Alharassi, Ahmed Suleiman
AU - Guillemin, Gilles
AU - Yamauchi, Motohiro
AU - Arivazhagan, Girija
AU - Song, Hyuk
PY - 2014
Y1 - 2014
N2 - Ionising radiation (γ or X-rays) is the mandatory tool to treat cancer despite its detrimental effects in particular on skin cells which lead to severe dermatological diseases and carcinogenesis. Natural antioxidants caffeic acid (CA), rosmarinic acid (RA), trans cinnamic acid (TCA), p-coumaric acid (PCA), and hydroxyphenyllactic acid (HPA) acid are known to be potent anticancer and antioxidant agents. Current study is designed to provide experimental evidence as these compounds offer radiation protection for skin cells. Non-toxic concentrations of CA, RA, TCA, PCA, and HPA were tested for radiation protection, γ-radiation induced intracellular reactive oxygen species (ROS) by flow cytometry and DNA double strand break in human keratinocytes (HaCaT cells) by immunocytochemistry. CA, RA and TCA pretreatment can protect the HaCaT cells by 40%, 20%, 15% respectively through scavenging γ-radiation induced ROS and decreasing number of post irradiation 53bp1 foci. Inclusion of these compounds in chemo-radiotherapy could facilitate to achieve multiple target protection (i.e. anti-cancer and skin radio protectant).
AB - Ionising radiation (γ or X-rays) is the mandatory tool to treat cancer despite its detrimental effects in particular on skin cells which lead to severe dermatological diseases and carcinogenesis. Natural antioxidants caffeic acid (CA), rosmarinic acid (RA), trans cinnamic acid (TCA), p-coumaric acid (PCA), and hydroxyphenyllactic acid (HPA) acid are known to be potent anticancer and antioxidant agents. Current study is designed to provide experimental evidence as these compounds offer radiation protection for skin cells. Non-toxic concentrations of CA, RA, TCA, PCA, and HPA were tested for radiation protection, γ-radiation induced intracellular reactive oxygen species (ROS) by flow cytometry and DNA double strand break in human keratinocytes (HaCaT cells) by immunocytochemistry. CA, RA and TCA pretreatment can protect the HaCaT cells by 40%, 20%, 15% respectively through scavenging γ-radiation induced ROS and decreasing number of post irradiation 53bp1 foci. Inclusion of these compounds in chemo-radiotherapy could facilitate to achieve multiple target protection (i.e. anti-cancer and skin radio protectant).
UR - http://www.scopus.com/inward/record.url?scp=84904458613&partnerID=8YFLogxK
U2 - 10.1504/IJLR.2014.063414
DO - 10.1504/IJLR.2014.063414
M3 - Article
AN - SCOPUS:84904458613
VL - 9
SP - 305
EP - 316
JO - International Journal of Low Radiation
JF - International Journal of Low Radiation
SN - 1477-6545
IS - 4
ER -