An inter-island comparison of Darwin’s finches reveals the impact of habitat, host phylogeny, and island on the gut microbiome

Wesley T. Loo, Rachael Y. Dudaniec, Sonia Kleindorfer, Colleen M. Cavanaugh

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Darwin’s finch species in the Galapagos Archipelago are an iconic adaptive radiation that offer a natural experiment to test for the various factors that influence gut microbiome composition. The island of Floreana has the longest history of human settlement within the archipelago and offers an opportunity to compare island and habitat effects on Darwin’s finch microbiomes. In this study, we compare gut microbiomes in Darwin’s finch species on Floreana Island to test for effects of host phylogeny, habitat (lowlands, highlands), and island (Floreana, Santa Cruz). We used 16S rRNA Illumina sequencing of fecal samples to assess the gut microbiome composition of Darwin’s finches, complemented by analyses of stable isotope values and foraging data to provide ecological context to the patterns observed. Overall bacterial composition of the gut microbiome demonstrated co-phylogeny with Floreana hosts, recapitulated the effect of habitat and diet, and showed differences across islands. The finch phylogeny uniquely explained more variation in the microbiome than did foraging data. Finally, there were interaction effects for island × habitat, whereby the same Darwin’s finch species sampled on two islands differed in microbiome for highland samples (highland finches also had different diets across islands) but not lowland samples (lowland finches across islands had comparable diet). Together, these results corroborate the influence of phylogeny, age, diet, and sampling location on microbiome composition and emphasize the necessity for comprehensive sampling given the multiple factors that influence the gut microbiome in Darwin’s finches, and by extension, in animals broadly.

LanguageEnglish
Article numbere0226432
Pages1-24
Number of pages24
JournalPLoS ONE
Volume14
Issue number12
DOIs
Publication statusPublished - 13 Dec 2019

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Finches
Phylogeny
Nutrition
Islands
Ecosystem
digestive system
phylogeny
habitats
Chemical analysis
Microbiota
Sampling
Diet
lowlands
highlands
Isotopes
diet
Animals
sampling
Radiation
foraging

Bibliographical note

Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Cite this

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abstract = "Darwin’s finch species in the Galapagos Archipelago are an iconic adaptive radiation that offer a natural experiment to test for the various factors that influence gut microbiome composition. The island of Floreana has the longest history of human settlement within the archipelago and offers an opportunity to compare island and habitat effects on Darwin’s finch microbiomes. In this study, we compare gut microbiomes in Darwin’s finch species on Floreana Island to test for effects of host phylogeny, habitat (lowlands, highlands), and island (Floreana, Santa Cruz). We used 16S rRNA Illumina sequencing of fecal samples to assess the gut microbiome composition of Darwin’s finches, complemented by analyses of stable isotope values and foraging data to provide ecological context to the patterns observed. Overall bacterial composition of the gut microbiome demonstrated co-phylogeny with Floreana hosts, recapitulated the effect of habitat and diet, and showed differences across islands. The finch phylogeny uniquely explained more variation in the microbiome than did foraging data. Finally, there were interaction effects for island × habitat, whereby the same Darwin’s finch species sampled on two islands differed in microbiome for highland samples (highland finches also had different diets across islands) but not lowland samples (lowland finches across islands had comparable diet). Together, these results corroborate the influence of phylogeny, age, diet, and sampling location on microbiome composition and emphasize the necessity for comprehensive sampling given the multiple factors that influence the gut microbiome in Darwin’s finches, and by extension, in animals broadly.",
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An inter-island comparison of Darwin’s finches reveals the impact of habitat, host phylogeny, and island on the gut microbiome. / Loo, Wesley T.; Dudaniec, Rachael Y.; Kleindorfer, Sonia; Cavanaugh, Colleen M.

In: PLoS ONE, Vol. 14, No. 12, e0226432, 13.12.2019, p. 1-24.

Research output: Contribution to journalArticleResearchpeer-review

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