An Mr 29 000 protein is essential for mini-F maintenance in E. coli

L. A. Watson*, S. H. Phua, P. L. Bergquist, H. E D Lane

*Corresponding author for this work

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32 Citations (Scopus)

Abstract

Plasmids consisting of mini-F inserted into multicopy vectors were constructed. Derivatives of these hybrid replicons were isolated which contained the transposon Tn5. The polypeptides encoded by these plasmids were identified by Escherichia coli minicell analysis. We show that a previously unidentified polypeptide of 29000 Mr is encoded by the mini-F gene E between 45.1 and 46.2 F kb on the mini-F plasmid map, and that this coding sequence (E gene) is transcribed rightward. Hybrid plasmids carrying Tn5 inserted into the E gene are unable to replicate in a polA- strain. Hence the E protein is essential for mini-F replication. Mutations in the A and B genes of mini-F affect E gene expression, and the results suggest that E protein synthesis is stimulated by A protein.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalGene
Volume19
Issue number2
DOIs
Publication statusPublished - 1982
Externally publishedYes

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Keywords

  • Escherichia coli plasmid
  • F replication
  • minicells
  • replication in polA hosts
  • restrictionanalysis
  • transposon Tn5

Cite this

Watson, L. A., Phua, S. H., Bergquist, P. L., & Lane, H. E. D. (1982). An Mr 29 000 protein is essential for mini-F maintenance in E. coli. Gene, 19(2), 173-178. https://doi.org/10.1016/0378-1119(82)90003-8