An Mr 29 000 protein is essential for mini-F maintenance in E. coli

L. A. Watson; S. H. Phua; P. L. Bergquist; H. E D Lane

doi:10.1016/0378-1119(82)90003-8

An Mr 29 000 protein is essential for mini-F maintenance in E. coli

L. A. Watson^*, S. H. Phua, P. L. Bergquist, H. E D Lane

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Plasmids consisting of mini-F inserted into multicopy vectors were constructed. Derivatives of these hybrid replicons were isolated which contained the transposon Tn5. The polypeptides encoded by these plasmids were identified by Escherichia coli minicell analysis. We show that a previously unidentified polypeptide of 29000 M_r is encoded by the mini-F gene E between 45.1 and 46.2 F kb on the mini-F plasmid map, and that this coding sequence (E gene) is transcribed rightward. Hybrid plasmids carrying Tn5 inserted into the E gene are unable to replicate in a polA^- strain. Hence the E protein is essential for mini-F replication. Mutations in the A and B genes of mini-F affect E gene expression, and the results suggest that E protein synthesis is stimulated by A protein.

Original language	English
Pages (from-to)	173-178
Number of pages	6
Journal	Gene
Volume	19
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(82)90003-8
Publication status	Published - 1982
Externally published	Yes

Keywords

Escherichia coli plasmid
F replication
minicells
replication in polA hosts
restrictionanalysis
transposon Tn5

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10.1016/0378-1119(82)90003-8

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title = "An Mr 29 000 protein is essential for mini-F maintenance in E. coli",

abstract = "Plasmids consisting of mini-F inserted into multicopy vectors were constructed. Derivatives of these hybrid replicons were isolated which contained the transposon Tn5. The polypeptides encoded by these plasmids were identified by Escherichia coli minicell analysis. We show that a previously unidentified polypeptide of 29000 Mr is encoded by the mini-F gene E between 45.1 and 46.2 F kb on the mini-F plasmid map, and that this coding sequence (E gene) is transcribed rightward. Hybrid plasmids carrying Tn5 inserted into the E gene are unable to replicate in a polA- strain. Hence the E protein is essential for mini-F replication. Mutations in the A and B genes of mini-F affect E gene expression, and the results suggest that E protein synthesis is stimulated by A protein.",

keywords = "Escherichia coli plasmid, F replication, minicells, replication in polA hosts, restrictionanalysis, transposon Tn5",

author = "Watson, \{L. A.\} and Phua, \{S. H.\} and Bergquist, \{P. L.\} and Lane, \{H. E D\}",

year = "1982",

doi = "10.1016/0378-1119(82)90003-8",

language = "English",

volume = "19",

pages = "173--178",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - An Mr 29 000 protein is essential for mini-F maintenance in E. coli

AU - Watson, L. A.

AU - Phua, S. H.

AU - Bergquist, P. L.

AU - Lane, H. E D

PY - 1982

Y1 - 1982

N2 - Plasmids consisting of mini-F inserted into multicopy vectors were constructed. Derivatives of these hybrid replicons were isolated which contained the transposon Tn5. The polypeptides encoded by these plasmids were identified by Escherichia coli minicell analysis. We show that a previously unidentified polypeptide of 29000 Mr is encoded by the mini-F gene E between 45.1 and 46.2 F kb on the mini-F plasmid map, and that this coding sequence (E gene) is transcribed rightward. Hybrid plasmids carrying Tn5 inserted into the E gene are unable to replicate in a polA- strain. Hence the E protein is essential for mini-F replication. Mutations in the A and B genes of mini-F affect E gene expression, and the results suggest that E protein synthesis is stimulated by A protein.

AB - Plasmids consisting of mini-F inserted into multicopy vectors were constructed. Derivatives of these hybrid replicons were isolated which contained the transposon Tn5. The polypeptides encoded by these plasmids were identified by Escherichia coli minicell analysis. We show that a previously unidentified polypeptide of 29000 Mr is encoded by the mini-F gene E between 45.1 and 46.2 F kb on the mini-F plasmid map, and that this coding sequence (E gene) is transcribed rightward. Hybrid plasmids carrying Tn5 inserted into the E gene are unable to replicate in a polA- strain. Hence the E protein is essential for mini-F replication. Mutations in the A and B genes of mini-F affect E gene expression, and the results suggest that E protein synthesis is stimulated by A protein.

KW - Escherichia coli plasmid

KW - F replication

KW - minicells

KW - replication in polA hosts

KW - restrictionanalysis

KW - transposon Tn5

UR - https://www.scopus.com/pages/publications/0020334380

U2 - 10.1016/0378-1119(82)90003-8

DO - 10.1016/0378-1119(82)90003-8

M3 - Article

C2 - 6293926

AN - SCOPUS:0020334380

SN - 0378-1119

VL - 19

SP - 173

EP - 178

JO - Gene

JF - Gene

IS - 2

ER -

An Mr 29 000 protein is essential for mini-F maintenance in E. coli

Abstract

Keywords

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