An oxygen-consuming phantom simulating perfused tissue to explore oxygen dynamics and 19F MRI oximetry

Steven H. Baete, Jan Vandecasteele, Luc Colman, Wilfried De Neve, Yves De Deene

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Objective: This study presents a reproducible phantom which mimics oxygen-consuming tissue and can be used for the validation of 19F MRI oximetry. Materials and methods: The phantom consists of a haemodialysis filter of which the outer compartment is filled with a gelatin matrix containing viable yeast cells. Perfluorocarbon emulsions can be added to the gelatin matrix to simulate sequestered perfluorocarbons. A blood-substituting perfluorocarbon fluid is pumped through the lumen of the fibres in the filter. 19F relaxometry MRI is performed with a fast 2D Look-Locker imaging sequence on a clinical 3T scanner. Results: Acute and perfusion-related hypoxia were simulated and imaged spatially and temporally using the phantom. Conclusions: The presented experimental setup can be used to simulate oxygen consumption by somatic cells in vivo and for validating computational biophysical models of hypoxia, as measured with 19F MRI oximetry.

Original languageEnglish
Pages (from-to)217-226
Number of pages10
JournalMagnetic Resonance Materials in Physics, Biology and Medicine
Volume23
Issue number4
DOIs
Publication statusPublished - Sep 2010
Externally publishedYes

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