Analysis of a transfer region from the staphylococcal conjugative plasmid pSK41

Neville Firth, Karyn P. Ridgway, Mary E. Byrne, Peter D. Fink, Luke Johnson, Ian T. Paulsen, Ronald A. Skurray*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


The nucleotide sequence of a 14.4-kb region (tra) associated with DNA transfer of the staphylococcal conjugative plasmid, pSK41, has been determined. Analysis of the sequence revealed the presence of 15 genes potentially involved in the conjugative process. Polypeptide products likely to correspond to ten of these genes have been identified, of which one was found to be a lipoprotein. Comparison of the deduced tra products to the protein databases revealed several interesting similarities, one of which suggests an evolutionary link between this Gram+ bacterial conjugation system and DNA transfer systems of Gram- bacteria, such as Escherichia coli and Agrobacterium tumefaciens. The nt sequence also provided an insight into the transcriptional organisation and regulation of the region.

Original languageEnglish
Pages (from-to)13-25
Number of pages13
Issue number1-2
Publication statusPublished - 22 Dec 1993
Externally publishedYes


  • Conjugation
  • DNA transfer
  • lipoprotein
  • membrane proteins
  • pheromone
  • topoisomerase


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