Analysis of a transfer region from the staphylococcal conjugative plasmid pSK41

Neville Firth; Karyn P. Ridgway; Mary E. Byrne; Peter D. Fink; Luke Johnson; Ian T. Paulsen; Ronald A. Skurray

doi:10.1016/0378-1119(93)90442-6

Analysis of a transfer region from the staphylococcal conjugative plasmid pSK41

Neville Firth, Karyn P. Ridgway, Mary E. Byrne, Peter D. Fink, Luke Johnson, Ian T. Paulsen, Ronald A. Skurray^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The nucleotide sequence of a 14.4-kb region (tra) associated with DNA transfer of the staphylococcal conjugative plasmid, pSK41, has been determined. Analysis of the sequence revealed the presence of 15 genes potentially involved in the conjugative process. Polypeptide products likely to correspond to ten of these genes have been identified, of which one was found to be a lipoprotein. Comparison of the deduced tra products to the protein databases revealed several interesting similarities, one of which suggests an evolutionary link between this Gram⁺ bacterial conjugation system and DNA transfer systems of Gram^- bacteria, such as Escherichia coli and Agrobacterium tumefaciens. The nt sequence also provided an insight into the transcriptional organisation and regulation of the region.

Original language	English
Pages (from-to)	13-25
Number of pages	13
Journal	Gene
Volume	136
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(93)90442-6
Publication status	Published - 22 Dec 1993
Externally published	Yes

Keywords

Conjugation
DNA transfer
lipoprotein
membrane proteins
pheromone
topoisomerase

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10.1016/0378-1119(93)90442-6

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title = "Analysis of a transfer region from the staphylococcal conjugative plasmid pSK41",

abstract = "The nucleotide sequence of a 14.4-kb region (tra) associated with DNA transfer of the staphylococcal conjugative plasmid, pSK41, has been determined. Analysis of the sequence revealed the presence of 15 genes potentially involved in the conjugative process. Polypeptide products likely to correspond to ten of these genes have been identified, of which one was found to be a lipoprotein. Comparison of the deduced tra products to the protein databases revealed several interesting similarities, one of which suggests an evolutionary link between this Gram+ bacterial conjugation system and DNA transfer systems of Gram- bacteria, such as Escherichia coli and Agrobacterium tumefaciens. The nt sequence also provided an insight into the transcriptional organisation and regulation of the region.",

keywords = "Conjugation, DNA transfer, lipoprotein, membrane proteins, pheromone, topoisomerase",

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T1 - Analysis of a transfer region from the staphylococcal conjugative plasmid pSK41

AU - Firth, Neville

AU - Ridgway, Karyn P.

AU - Byrne, Mary E.

AU - Fink, Peter D.

AU - Johnson, Luke

AU - Paulsen, Ian T.

AU - Skurray, Ronald A.

PY - 1993/12/22

Y1 - 1993/12/22

N2 - The nucleotide sequence of a 14.4-kb region (tra) associated with DNA transfer of the staphylococcal conjugative plasmid, pSK41, has been determined. Analysis of the sequence revealed the presence of 15 genes potentially involved in the conjugative process. Polypeptide products likely to correspond to ten of these genes have been identified, of which one was found to be a lipoprotein. Comparison of the deduced tra products to the protein databases revealed several interesting similarities, one of which suggests an evolutionary link between this Gram+ bacterial conjugation system and DNA transfer systems of Gram- bacteria, such as Escherichia coli and Agrobacterium tumefaciens. The nt sequence also provided an insight into the transcriptional organisation and regulation of the region.

AB - The nucleotide sequence of a 14.4-kb region (tra) associated with DNA transfer of the staphylococcal conjugative plasmid, pSK41, has been determined. Analysis of the sequence revealed the presence of 15 genes potentially involved in the conjugative process. Polypeptide products likely to correspond to ten of these genes have been identified, of which one was found to be a lipoprotein. Comparison of the deduced tra products to the protein databases revealed several interesting similarities, one of which suggests an evolutionary link between this Gram+ bacterial conjugation system and DNA transfer systems of Gram- bacteria, such as Escherichia coli and Agrobacterium tumefaciens. The nt sequence also provided an insight into the transcriptional organisation and regulation of the region.

KW - Conjugation

KW - DNA transfer

KW - lipoprotein

KW - membrane proteins

KW - pheromone

KW - topoisomerase

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VL - 136

SP - 13

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ER -

Analysis of a transfer region from the staphylococcal conjugative plasmid pSK41

Abstract

Keywords

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